ArticleScientific reports2017
Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 76 citations in OpenAlex.
- Variable partition of non-homogeneous ooplasm sets the stage for divergent potency of 2-cell stage blastomeres.Molecular human reproduction · 2026Article
- Article
- Role of CEBPa in trophectoderm competence installment.Science advances · 2025Article
- Expression of LINE-1 elements is required for preimplantation development and totipotency.Genes & diseases · 2025Review
- Totipotency or plenipotency: rethinking stem cell bipotentiality.Current opinion in genetics & development · 2025Review
- Male gamete copies to characterize genome inheritance and generate progenies.Scientific reports · 2025Article
- The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision.eLife · 2025Article
- Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start.Molecular human reproduction · 2025Article
- Cellular mechanisms of monozygotic twinning: clues from assisted reproduction.Human reproduction update · 2024Review
- The role of lipids in genome integrity and pluripotency.Biochemical Society transactions · 2024Review
- Endogenous retroviruses shape pluripotency specification in mouse embryos.Science advances · 2024Article
- A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models.Frontiers in cell and developmental biology · 2024Review
- Blastomere size in the human 2-cell embryo predicts the division order that leads to imbalanced lineage contribution to the future body.microPublication biology · 2024Article
- Intracellular fraction of zona pellucida protein 3 is required for the oocyte-to-embryo transition in mice.Molecular human reproduction · 2023Article
- Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.Frontiers in cell and developmental biology · 2023Article
- The role of polarization and early heterogeneities in the mammalian first cell fate decision.Current topics in developmental biology · 2023Review
- New insights into the epitranscriptomic control of pluripotent stem cell fate.Experimental & molecular medicine · 2022Review
- Asymmetric Contribution of Blastomere Lineages of First Division of the Zygote to Entire Human Body Using Post-Zygotic Variants.Tissue engineering and regenerative medicine · 2022Article
- Genome architecture and totipotency: An intertwined relation during early embryonic development.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Nucleolar-basedGenes & development · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following fertilization in mammals, it is generally accepted that totipotent cells are exclusive to the zygote and to each of the two blastomeres originating from the first mitotic division. This model of totipotency was inferred from a minority of cases in which blastomeres produced monozygotic twins in mice. Was this due to experimental limitation or biological constraint? Here we removed experimental obstacles and achieved reliable quantification of the prevalence of dual totipotency among mouse two-cell stage blastomeres. We separated the blastomeres of 1,252 two-cell embryos, preserving 1,210 of the pairs. Two classes of monozygotic twins became apparent at the blastocyst stage: 27% formed a functional epiblast in both members (concordant), and 73% did so in only one member of the pair (discordant) - a partition that proved insensitive to oocyte quality, sperm-entry point, culture environment and pattern of cleavage. In intact two-cell embryos, the ability of sister blastomeres to generate epiblast was also skewed. Class discovery clustering of the individual blastomeres' and blastocysts' transcriptomes points to an innate origin of concordance and discordance rather than developmental acquisition. Our data place constraints on the commonly accepted idea that totipotency is allocated equally between the two-cell stage blastomeres in mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.