Evidence map›Paper›PMID 28811502›Full record

ArticleScientific reports2017

Sex Steroid Hormones Regulate Leptin Transcript Accumulation and Protein Secretion in 3T3-L1 Cells.

Mónica Z Jenks, Heather E Fairfield, Erik C Johnson, Ron F Morrison, Gloria K Muday

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 2 pooled it
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 54 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Mónica Z JenksDepartment of Biology and Center for Molecular Signaling, Wake Forest University, Winston Salem, North Carolina, USA.
Heather E FairfieldDepartment of Biology and Center for Molecular Signaling, Wake Forest University, Winston Salem, North Carolina, USA.
Erik C JohnsonDepartment of Biology and Center for Molecular Signaling, Wake Forest University, Winston Salem, North Carolina, USA.
Ron F MorrisonDepartment of Nutrition, University of North Carolina at Greensboro, Greensboro, North Carolina, USA.
Gloria K MudayDepartment of Biology and Center for Molecular Signaling, Wake Forest University, Winston Salem, North Carolina, USA. muday@wfu.edu.
Wake Forest University · USUniversity of North Carolina at Greensboro · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Leptin is an adipokine produced by fat cells that regulates food consumption and metabolic activity. Sexual dimorphism in leptin and fat stores have been observed in humans and rodents with females having more leptin and greater levels of subcutaneous fat than males. One potential mechanism leading to this dimorphism is steroid hormone regulated synthesis of transcripts encoding leptin. Identification of direct regulatory mechanisms is difficult in animals or primary adipocytes due to these intertwined dimorphisms. We used well-characterized 3T3-L1 murine adipocytes to demonstrate that dihydrotestosterone (DHT) reduced Leptin (Lep) transcript abundance and cytosolic and secreted leptin protein. The magnitude of this effect was greatest on secreted leptin, which was decreased by DHT to 30% of the control. In contrast, 17β-estradiol significantly increased the abundance of transcripts encoding leptin and increased secreted leptin to 230% of the control. Treatment with estrogen and androgen receptor antagonists had opposite effects on Lep transcript abundance to steroid treatments, indicating that these transcriptional effects are mediated through the canonical steroid hormone signaling pathways. These results indicate that short-term treatments with steroid hormones are sufficient to alter both Lep transcript accumulation and leptin protein secretion, and may play a role in the sexual dimorphism of this adipokine.

Indexed as

Transcription, Genetic3T3-L1 CellsAndrogen Receptor AntagonistsAnimalsCells, CulturedDihydrotestosteroneEstradiolGene Expression RegulationGonadal Steroid HormonesLeptinMiceAndrogen Receptor AntagonistsDihydrotestosteroneEstradiolGonadal Steroid HormonesLeptin

Identifiers

PMID28811502
PMCPMC5558017
OpenAlexW2742282263

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.