ArticleMicrobial cell factories2017
New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 111 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Synthesis of Plant-InspiredACS synthetic biology · 2026Article
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Engineering de novo formatotrophy in the non-model yeastNature synthesis · 2026Article
- Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance.Nucleic acids research · 2025Article
- Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools.Microbial biotechnology · 2025Review
- New Yarrowia lipolytica chassis strains for industrial enzyme production.Microbial cell factories · 2025Article
- Efficient biosynthesis of β-caryophyllene by engineered Yarrowia lipolytica.Microbial cell factories · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- An Interplay between Transcription Factors and Recombinant Protein Synthesis inInternational journal of molecular sciences · 2024Article
- Screening of broad-host expression promoters for shuttle expression vectors in non-conventional yeasts and bacteria.Microbial cell factories · 2024Article
- 'Small volume-big problem': culturing Yarrowia lipolytica in high-throughput micro-formats.Microbial cell factories · 2024Review
- Review
- Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica.Microbial cell factories · 2024Article
- Using Euf1 transcription factor as a titrator of erythritol-inducible promoters in Yarrowia lipolytica; insight into the structure, splicing, and regulation mechanism.FEMS yeast research · 2024Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis inACS synthetic biology · 2023Article
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system.FEMS yeast research · 2023Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica.
resultsWe have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase. pEYK1 induction was found to be impaired in media supplemented with glucose and glycerol, while the presence of erythritol and erythrulose strongly increased the promoter induction level. Promoter characterization and mutagenesis allowed the identification of the upstream activating sequence UAS1
conclusionsNovel tunable and regulated promoters with applications in the field of heterologous protein production, metabolic engineering, and synthetic biology have been developed, thus filling the gap of the absence of versatile inducible promoter in the yeast Y. lipolytica.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.