ArticleBMC veterinary research2017
Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular detection of shared pathogens in red foxes (One health (Amsterdam, Netherlands) · 2026Article
- Circulation of arctic canine distemper virus in diarrheic dogs in Iran: molecular detection and phylogenetic characterization.BMC research notes · 2026Article
- Retrospective analysis of possible associations between Pneumocystis spp. and five immunosuppressive viral pathogens in three wild carnivore species.BMC veterinary research · 2026Article
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Investigation of the canine distemper virus in dogs living at public kennels in Northern Brazil: validation of a detection assay.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Article
- Molecular analysis of canine distemper virus H gene in the golden jackal (Canis aureus) population from Serbia.BMC veterinary research · 2024Article
- Recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) designed for rapid detection of canine distemper virus.The Journal of veterinary medical science · 2024Article
- Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms.Biosensors · 2024Review
- Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV).Frontiers in veterinary science · 2024Article
- Molecular epidemiological investigation and recombination analysis of Cachavirus prevalent in China.Frontiers in veterinary science · 2024Article
- Article
- Retrospective Detection and Phylogenetic Analysis of Cachavirus-Related Parvoviruses in Dogs in China.Transboundary and emerging diseases · 2023Article
- Molecular Characterization of Feline Chaphamaparvovirus (Transboundary and emerging diseases · 2023Article
- Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays.BMC veterinary research · 2022Article
- Canine morbillivirus (CDV): a review on current status, emergence and the diagnostics.Virusdisease · 2022Review
- Detection of canine distemper virus in cerebrospinal fluid, whole blood and mucosal specimens of dogs with distemper using RT-PCR and immunochromatographic assays.Veterinary medicine and science · 2022Article
- Development of a Real-Time Recombinase Polymerase Amplification Assay for the Rapid Detection of African Swine Fever Virus Genotype I and II.Pathogens (Basel, Switzerland) · 2022Article
- Article
- Rapid, Simple, and Highly Specific Detection ofFrontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCanine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens.
methodsA real-time reverse transcription RPA (RT-RPA) assay for the detection of canine distemper virus (CDV) using primers and exo probe targeting the CDV nucleocapsid protein gene was developed. A series of other viruses were tested by the RT-RPA.Thirty-two field samples were further tested by RT-RPA, and the resuts were compared with those obtained by the real-time RT-PCR.
resultsThe RT-RPA assay was performed successfully at 40 °C, and the results were obtained within 3 min-12 min. The assay could detect CDV, but did not show cross-detection of canine parvovirus-2 (CPV-2), canine coronavirus (CCoV), canine parainfluenza virus (CPIV), pseudorabies virus (PRV) or Newcastle disease virus (NDV), demonstrating high specificity. The analytical sensitivity of RT-RPA was 31.8 copies in vitro transcribed CDV RNA, which is 10 times lower than the real-time RT-PCR. The assay performance was validated by testing 32 field samples and compared to real-time RT-PCR. The results indicated an excellent correlation between RT-RPA and a reference real-time RT-PCR method. Both assays provided the same results, and R
conclusionsThe results demonstrated that the RT-RPA assay offers an alternative tool for simple, rapid, and reliable detection of CDV both in the laboratory and point-of-care facility, especially in the resource-limited settings.
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