Evidence map›Paper›PMID 28809547›Full record

ArticleAnalytical chemistry2017

Quantitative Evaluation of Viral Protein Binding to Phosphoinositide Receptors and Pharmacological Inhibition.

Seong-Oh Kim, Joshua A Jackman, Menashe Elazar, Sang-Joon Cho, Jeffrey S Glenn, Nam-Joon Cho

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Seong-Oh KimSchool of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Drive, 637553 Singapore.
Joshua A JackmanSchool of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Drive, 637553 Singapore.
Menashe ElazarDivision of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine , Stanford, California 94305, United States.
Sang-Joon ChoAdvanced Institute of Convergence Technology, Seoul National University , Suwon 443-270, South Korea.
Jeffrey S GlennDivision of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine , Stanford, California 94305, United States.
Nam-Joon ChoSchool of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Drive, 637553 Singapore.ORCID 0000-0002-8692-8955

Funding

Translational Incubator CoreU19AI109662 · NIAID · STANFORD UNIVERSITY · PI GLENN, JEFFREY S · 2014 to 2019
$28.6M
The PIP2-virus interface and PI 4-kinase: novel biology and validation targetsR01AI099245 · NIAID · STANFORD UNIVERSITY · PI GLENN, JEFFREY S · 2012 to 2016
$5.2M
NIAID NIH HHS R01 AI099245NIAID NIH HHS U19 AI109662
6 · The paper itself

Abstract

There is significant interest in developing analytical methods to characterize molecular recognition events between proteins and phosphoinositides, which are a medically important class of carbohydrate-functionalized lipids. Within this scope, one area of high priority involves quantitatively evaluating drug candidates that pharmacologically inhibit protein-phosphoinositide interactions. As full-length proteins are often difficult to produce, establishing methods to study these interactions with shorter, bioactive peptides would be advantageous. Herein, we report an atomic force microscopy (AFM)-based force spectroscopic approach to detect the specific interaction between an amphipathic, α-helical (AH) peptide derived from the hepatitis C virus NS5A protein and its biological target, the phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P

Indexed as

Dose-Response Relationship, DrugMicroscopy, Atomic ForceNeomycinProtein BindingProtein Synthesis InhibitorsReceptors, Cytoplasmic and NuclearRNA-Dependent RNA PolymeraseStructure-Activity RelationshipViral Nonstructural ProteinsNeomycinNS-5 protein, hepatitis C virusphosphatidylinositol receptorsProtein Synthesis InhibitorsReceptors, Cytoplasmic and NuclearRNA-Dependent RNA PolymeraseViral Nonstructural Proteins

Identifiers

PMID28809547
PMCPMC5724528

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.