Evidence map›Paper›PMID 28809165›Full record

ReviewMethods and applications in fluorescence2016

From single-molecule spectroscopy to super-resolution imaging of the neuron: a review.

Romain F Laine, Gabriele S Kaminski Schierle, Sebastian van de Linde, Clemens F Kaminski

Abstract readReview
In one paragraph

Review in Methods and applications in fluorescence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Review
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  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Romain F LaineLaser Analytics Group, Department of Chemical Engineering and Biotechnology, Cambridge University, Pembroke Street, Cambridge, CB2 3RA, UK.
Gabriele S Kaminski Schierle
Sebastian van de Linde
Clemens F Kaminski

Funding

Medical Research Council MC_G1000734Medical Research Council MR/K015850/1Medical Research Council MR/K02292X/1Wellcome Trust
6 · The paper itself

Abstract

For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.

Indexed as

NeuronsFluorescent DyesImaging, Three-DimensionalMacromolecular SubstancesMicroscopyMicroscopy, FluorescenceMolecular ImagingNanotechnologyOptical ImagingSpectrum AnalysisFluorescent DyesMacromolecular Substances

Identifiers

PMID28809165
PMCPMC5390958

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.