Evidence map›Paper›PMID 28809003›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy.

A Cameron Varano, Naoe Harafuji, William Dearnaley, Lisa Guay-Woodford, Deborah F Kelly

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Differential regulation of MYC expression byFrontiers in cell and developmental biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

A Cameron VaranoVirginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, VA, 24016, USA.
Naoe HarafujiCenter for Translational Science, Children's National Health System, Washington, DC, 20010, USA.
William DearnaleyVirginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, VA, 24016, USA.
Lisa Guay-WoodfordCenter for Translational Science, Children's National Health System, Washington, DC, 20010, USA.
Deborah F KellyVirginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, VA, 24016, USA. debkelly@vt.edu.

Funding

Tunable Microchip Sorting of BRCA1 Nuclear AssembliesR01CA193578 · NCI · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI KELLY, DEBORAH F · 2015 to 2020
$1.8M
NCI NIH HHS R01 CA193578
6 · The paper itself

Abstract

Electron microscopy (EM) is a rapidly growing area of structural biology that permits us to decode biological assemblies at the nanoscale. To examine biological materials for single particle EM analysis, purified assemblies must be obtained using biochemical separation techniques. Here, we describe effective methodologies for isolating histidine (his)-tagged protein assemblies from the nucleus of disease-relevant cell lines. We further demonstrate how isolated assemblies are visualized using single particle EM techniques and provide representative results for each step in the process.

Indexed as

Analytic Sample Preparation MethodsAnimalsCell LineChromatography, AffinityDrug DiscoveryHistidineHumansMiceMicroscopy, ElectronModels, MolecularOrganellesOrganometallic CompoundsReceptors, Cell SurfaceSingle Molecule ImagingHistidineOrganometallic CompoundsPKHD1 protein, humanReceptors, Cell Surfaceuranyl formateElectron microscopyFibrocystinHistidine tagRecombinant proteinsSingle particle analysis

Identifiers

PMID28809003
PMCPMC5609838

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.