Evidence map›Paper›PMID 28808993›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Multiplexed Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry Quantification of Cancer Signaling Proteins.

Yi Chen, Kate J Fisher, Mark Lloyd, Elizabeth R Wood, Domenico Coppola, Erin Siegel, David Shibata, Yian A Chen, John M Koomen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Cell Type-specific Adaptive Signaling Responses to KRASClinical cancer research : an official journal of the American Association for Cancer Research · 2021
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Yi ChenH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Kate J FisherH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Mark LloydH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Elizabeth R WoodH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Domenico CoppolaH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Erin SiegelH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
David ShibataH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
Yian A ChenH. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.
John M KoomenMolecular Oncology and Proteomics, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, 12902 Magnolia Drive, Tampa, FL, 33612, USA. john.koomen@moffitt.org.
University of South Florida · USMoffitt Cancer Center · US

Funding

TRANSLATIONAL RESEARCHP30CA076292 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI Christine O'Connell · 1998 to 2026
$93.5M
NCI NIH HHS P30 CA076292
6 · The paper itself

Abstract

Quantitative evaluation of protein expression across multiple cancer-related signaling pathways (e.g., Wnt/β-catenin, TGF-β, receptor tyrosine kinases (RTK), MAP kinases, NF-κB, and apoptosis) in tumor tissues may enable the development of a molecular profile for each individual tumor that can aid in the selection of appropriate targeted cancer therapies. Here, we describe the development of a broadly applicable protocol to develop and implement quantitative mass spectrometry assays using cell line models and frozen tissue specimens from colon cancer patients. Cell lines are used to develop peptide-based assays for protein quantification, which are incorporated into a method based on SDS-PAGE protein fractionation, in-gel digestion, and liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM/MS). This analytical platform is then applied to frozen tumor tissues. This protocol can be broadly applied to the study of human disease using multiplexed LC-MRM assays.

Indexed as

Protein Interaction MapsBiomarkers, TumorCell Line, TumorChromatography, LiquidColonic NeoplasmsHumansMass SpectrometryNeoplasm ProteinsPeptidesProteomicsSignal TransductionBiomarkers, TumorNeoplasm ProteinsPeptidesCancerColorectal carcinomaLiquid chromatography-multiple reaction monitoring mass spectrometryProtein expressionSignalingTargeted quantification

Identifiers

PMID28808993
PMCPMC5729929
OpenAlexW2746873371

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.