Evidence map›Paper›PMID 28808962›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes.

Tianyun Long, Rui Lu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Small RNA-based antimicrobial immunity.Nature reviews. Immunology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Tianyun LongDepartment of Biological Sciences, Louisiana State University, 202 Life Science Building, Baton Rouge, LA, 70803, USA.
Rui LuDepartment of Biological Sciences, Louisiana State University, 202 Life Science Building, Baton Rouge, LA, 70803, USA. ruilu@lsu.edu.
Louisiana State University · US

Funding

Cytosolic virus detection in Caenorhabditis elegansR56AI107249 · NIAID · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI LU, RUI · 2014 to 2014
$230k
NIAID NIH HHS R56 AI107249
6 · The paper itself

Abstract

Northern blot analysis has been widely used as a tool for detection and characterization of specific RNA molecules. When coupled with radioactive probe northern blot allows for robust detection and characterization of small RNA molecules of trace amount. Here, we describe the detection and size characterization of virus-derived small interfering RNAs (vsiRNAs) in C. elegans using nonradioactive DNA oligo probes in northern blotting. Our protocol allows for the detection and characterization of not only primary vsiRNAs but also secondary vsiRNAs, a class of single-stranded vsiRNAs that has distinct migration pattern, and can be easily adapted to the detection of vsiRNAs in other organisms.

Indexed as

Caenorhabditis elegansRNA, Small InterferingRNA, ViralAnimalsBlotting, NorthernOligodeoxyribonucleotidesOligodeoxyribonucleotidesRNA, Small InterferingRNA, ViralCaenorhabditis elegansDIG-labeled probeNonradioactiveNorthern blotPAGE gel electrophoresisVirus-derived siRNA

Identifiers

PMID28808962
OpenAlexW2749885512

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.