ArticleScientific reports2017
Rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of Gram-negative clinical isolates.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Microfluidic point-of-care testing for antimicrobial resistance: advances, strategies, and One Health perspectives.Science in One Health · 2026Review
- Next-Gen Nano Biosensor Technologies to Monitor Carbapenem Resistance for Personalized Medicine.Indian journal of microbiology · 2025Article
- Ribosome phenotypes for rapid classification of antibiotic-susceptible and resistant strains of Escherichia coli.Communications biology · 2025Article
- Investigation of cefiderocol resistance prevalence and resistance mechanisms in carbapenem-resistantJAC-antimicrobial resistance · 2024Article
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Growth Independent Morphometric Machine Learning Workflow for Single-Cell Antimicrobial Susceptibility Testing ofFrontiers in imaging (Lausanne, Switzerland) · 2024Article
- Effects of Oxytetracycline/Lead Pollution Alone and in the Combined Form on Antibiotic Resistance Genes, Mobile Genetic Elements, and Microbial Communities in the Soil.International journal of environmental research and public health · 2022Article
- Ti/PbOInternational journal of environmental research and public health · 2022Article
- The Spread of Antibiotic Resistance Genes In Vivo Model.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Review
- Biochar and Hyperthermophiles as Additives Accelerate the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements during Composting.Materials (Basel, Switzerland) · 2021Article
- Microfluidics-Based Organism Isolation from Whole Blood: An Emerging Tool for Bloodstream Infection Diagnosis.Annals of biomedical engineering · 2019Review
- Sequencing-based methods and resources to study antimicrobial resistance.Nature reviews. Genetics · 2019Review
- Optimization of Stress-Based Microfluidic Testing for Methicillin Resistance inDiagnostics (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. Since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both Gram-positive and Gram-negative bacterial species requires at least 48-72 h. Recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the microscopic growth analysis of small bacterial populations. These approaches are still inherently limited by the bacterial growth rate. Our approach is fundamentally different. By applying environmental stress to bacteria in a microfluidic platform, we can correctly assign antibiotic susceptibility profiles of clinically relevant Gram-negative bacteria within two hours of antibiotic introduction rather than 8-24 h. The substantial expansion to include a number of clinical isolates of important Gram-negative species-Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa-reported here underscores the broad utility of our approach, complementing the method's proven utility for Gram-positive bacteria. We also demonstrate that the platform is compatible with antibiotics that have varying mechanisms of action-meropenem, gentamicin, and ceftazidime-highlighting the versatility of this platform.
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