Evidence map›Paper›PMID 28808330›Full record

ArticleScientific reports2017

Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons.

Joseph D Dougherty

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Midbrain Dopaminergic Neuron Development at the Single Cell Level:Frontiers in cell and developmental biology · 2020
    Review
  3. The Expanding Toolkit of Translating Ribosome Affinity Purification.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Joseph D DoughertyDepartment of Genetics, Washington University School of Medicine, St. Louis, MO, USA. jdougherty@genetics.wustl.edu.
Washington University in St. Louis · US

Funding

Washington University Intellectual and Developmental Disabilities Research Center-Administrative Down Syndrome SupplementU54HD087011 · NICHD · WASHINGTON UNIVERSITY · PI CONSTANTINO, JOHN N. · 2015 to 2019
$7.1M
AN INDUCIBLE MOLECULAR MEMORY SYSTEM TO RECORD TRANSIENT STATES OF CNS CELLSU01MH109133 · NIMH · WASHINGTON UNIVERSITY · PI MITRA, ROBI D · 2015 to 2017
$3.6M
REGULATION OF MAMMALIAN SOCIAL BEHAVIOR BY THE GTF2I FAMILY OF PROTEINSR01MH107515 · NIMH · WASHINGTON UNIVERSITY · PI DOUGHERTY, JOSEPH D · 2016 to 2020
$2.4M
MASSIVELY PARALLEL CHARACTERIZATION OF CIS-REGULATORY ELEMENTS IN THE BRAINR01HG008687 · NHGRI · WASHINGTON UNIVERSITY · PI COHEN, BARAK A · 2015 to 2017
$1.1M
USING TRANSGENIC MICE TO EXAMINE THE ROLE OF CNIH3 VARIANTS IN OPIOID DEPENDENCER21DA041883 · NIDA · WASHINGTON UNIVERSITY · PI DOUGHERTY, JOSEPH D, MORON-CONCEPCION, JOSE A · 2016 to 2017
$379k
DEVELOPMENT OF A NOVEL APPROACH TO STUDY ALTERNATIVE TRANSLATION IN THE CNSR21DA038458 · NIDA · WASHINGTON UNIVERSITY · PI DOUGHERTY, JOSEPH D · 2014 to 2015
$378k
NHGRI NIH HHS R01 HG008687NICHD NIH HHS U54 HD087011NIDA NIH HHS R21 DA038458NIDA NIH HHS R21 DA041883NIMH NIH HHS R01 MH107515NIMH NIH HHS U01 MH109133
6 · The paper itself

Abstract

We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.

Indexed as

AnimalsDopamineDopaminergic NeuronsGene ExpressionMesencephalonMiceProtein BiosynthesisRibosomesRNA, MessengerDopamineRNA, Messenger

Identifiers

PMID28808330
PMCPMC5556054
OpenAlexW2742998843

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.