ArticleScientific reports2017
Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens.Molecular biotechnology · 2026Review
- CRISPR-based synthetic biology toolkit development inSynthetic and systems biotechnology · 2026Article
- EditingBiomolecules · 2026Review
- The S862C amino acid change in CpMrr1 confers fluconazole resistance inJAC-antimicrobial resistance · 2025Article
- Probing gene function inmSphere · 2024Article
- Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans.G3 (Bethesda, Md.) · 2023Article
- CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis.mSphere · 2022Article
- Engineered probiotics.Microbial cell factories · 2022Review
- Ten decadal advances in fungal biology leading towards human well-being.Fungal diversity · 2022Review
- Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Applications of CRISPR/Cas gene-editing technology in yeast and fungi.Archives of microbiology · 2021Article
- Enhancing the chemical transformation ofVirulence · 2021Article
- Hsp90 interaction networks in fungi-tools and techniques.FEMS yeast research · 2021Review
- Genetic Manipulation as a Tool to UnravelJournal of fungi (Basel, Switzerland) · 2021Review
- Recent Advances in Genome Editing Tools in Medical Mycology Research.Journal of fungi (Basel, Switzerland) · 2021Review
- Role ofJournal of fungi (Basel, Switzerland) · 2020Article
- Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.Microorganisms · 2020Review
- Polymorphic centromere locations in the pathogenic yeastGenome research · 2020Article
- Genetic Manipulation of a Lipolytic YeastMicroorganisms · 2020Article
- CRISPR-Based Genetic Manipulation ofFrontiers in genome editing · 2020Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida parapsilosis is one of the most common causes of candidiasis, particularly in the very young and the very old. Studies of gene function are limited by the lack of a sexual cycle, the diploid genome, and a paucity of molecular tools. We describe here the development of a plasmid-based CRISPR-Cas9 system for gene editing in C. parapsilosis. A major advantage of the system is that it can be used in any genetic background, which we showed by editing genes in 20 different isolates. Gene editing is carried out in a single transformation step. The CAS9 gene is expressed only when the plasmid is present, and it can be removed easily from transformed strains. There is theoretically no limit to the number of genes that can be edited in any strain. Gene editing is increased by homology-directed repair in the presence of a repair template. Editing by non-homologous end joining (NHEJ) also occurs in some genetic backgrounds. Finally, we used the system to introduce unique tags at edited sites.
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