Evidence map›Paper›PMID 28807016›Full record

ArticleBMC cancer2017

Endogenous controls of gene expression in N-methyl-N-nitrosourea-induced T-cell lymphoma in p53-deficient mice.

Xi Wu, Susu Liu, Jianjun Lyu, Shuya Zhou, Yanwei Yang, Chenfei Wang, Wenda Gu, Qin Zuo, Baowen Li, Changfa Fan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Xi WuDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Susu LiuDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Jianjun LyuNational Center for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, Beijing Economic-Technological Development Area, A8 Hongda Middle Street, Beijing, 100176, China.
Shuya ZhouDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Yanwei YangNational Center for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, Beijing Economic-Technological Development Area, A8 Hongda Middle Street, Beijing, 100176, China.
Chenfei WangDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Wenda GuDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Qin ZuoDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Baowen LiDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China.
Changfa FanDivision of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, No. 2 Tiantan Xili, Beijing, 100050, China. fancf@nifdc.org.cn.
National Institutes for Food and Drug Control · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundReal-time polymerase chain reaction (PCR) has become an increasingly important technique for gene expression profiling because it can provide insights into complex biological and pathological processes and be used to predict disease or treatment outcomes. Although normalized data are necessary for an accurate estimation of mRNA expression levels, several pieces of evidence suggest that the expression of so-called housekeeping genes is not stable. This study aimed to validate reference genes for the normalization of real-time PCR in an N-methyl-N-nitrosourea (MNU)-induced T-cell lymphoma mouse model.

methodsT-cell lymphomas were generated in p53-deficient mice by treatment with 37.5 mg/kg MNU. Thymus and spleen were identified as the primary target organs with the highest incidences of lymphomas. We analyzed the RNA expression levels of eight potential endogenous reference genes (Gapdh, Rn18s, Actb, Hprt, B2M, Rplp0, Gusb, Ctbp1). The expression stabilities of these reference genes were tested at different time points after MNU treatment using geNorm and NormFinder algorithms.

resultsA total of 65% of MNU-treated mice developed T-cell lymphomas, with the spleen and thymus as the major target organs. All candidate reference genes were amplified efficiently by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Gene stability evaluation after MNU treatment and during lymphomagenesis revealed that Ctbp1 and Rplp0 were the most stably expressed genes in the thymus and spleen, respectively. RT-PCR of thymus RNA using two additional sets of primer confirmed that Ctbp1 was the most stable of all the candidate reference genes.

conclusionsWe provided suitable endogenous controls for gene expression studies in the T-cell lymphoma model.

Indexed as

Gene Expression Regulation, NeoplasticGenes, EssentialAlcohol OxidoreductasesAnimalsDisease Models, AnimalDNA-Binding ProteinsLymphoma, T-CellMethylnitrosoureaMiceMice, KnockoutReal-Time Polymerase Chain ReactionRibosomal ProteinsTumor Suppressor Protein p53Alcohol OxidoreductasesC-terminal binding proteinDNA-Binding ProteinsMethylnitrosourearibosomal protein P0Ribosomal ProteinsTumor Suppressor Protein p53Lymphoma modelp53-deficient mouseQuantitative real-time PCRReference genes

Identifiers

PMID28807016
PMCPMC5557555
OpenAlexW2745640429

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.