ArticlePloS one2017
Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- Plant Growth Regulation in Cell and Tissue Culture In Vitro.Plants (Basel, Switzerland) · 2024Review
- Ethanol treatment enhances drought stress avoidance in cassava (Manihot esculenta Crantz).Plant molecular biology · 2022Article
- Field transcriptome analysis reveals a molecular mechanism for cassava-flowering in a mountainous environment in Southeast Asia.Plant molecular biology · 2022Article
- Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava.Plant molecular biology · 2022Article
- Cassava mosaic disease and its management in Southeast Asia.Plant molecular biology · 2022Review
- Agrobacterium-mediated cassava transformation for the Asian elite variety KU50.Plant molecular biology · 2022Article
- Suppressed expression of starch branching enzyme 1 and 2 increases resistant starch and amylose content and modifies amylopectin structure in cassava.Plant molecular biology · 2022Article
- Cassava breeding and agronomy in Asia: 50 years of history and future directions.Breeding science · 2020Article
- Intracellular localization of histone deacetylase HDA6 in plants.Journal of plant research · 2019Article
- Application of fluorescent and UV-Vis detection methods to profile antimicrobial activity of cassava tissues for an efficientPlant biotechnology (Tokyo, Japan) · 2019Article
- Acetic Acid Treatment Enhances Drought Avoidance in Cassava (Frontiers in plant science · 2019Article
- Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.Methods and protocols · 2018Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium, results demonstrated that using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC. Gene expression analyses revealed that FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall components in comparison to FEC on normal CIM media, whereas the gene expression associated with energy flux was not dramatically altered. It is hypothesized that we reprogram non-FEC to FEC under low nitrogen, potassium and phosphate and high vitamin B1. These findings were more effective in inducing FEC formation than the previous protocol. It might contribute to development of an efficient transformation strategy in cassava.
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