ArticleBiomaterials science2017
Dispersible oxygen microsensors map oxygen gradients in three-dimensional cell cultures.
Article in Biomaterials science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Quantitative Spatiotemporal Analysis of Intracellular Kinase Activity in Metastatic Breast Cancer Cells Using a Microfluidic-Based Lateral Diffusion Assay.Analytical chemistry · 2026Article
- Modeling of Biomechanical and Functional Parameters of Hydrogel-Cell Composites Fabricated by 3D Bioprinting Using AI-Supported Approach.Materials (Basel, Switzerland) · 2026Article
- An Accessible Platform to Quantify Oxygen Diffusion in Cell-Laden Hydrogels and Its Application to Alginate-Immobilized Pancreatic Beta Cells.Biotechnology and bioengineering · 2026Article
- GelMA Core-Shell Microgel Preparation Based on a Droplet Microfluidic Device for Three-Dimensional Tumor Ball Culture and Its Drug Testing.Molecules (Basel, Switzerland) · 2025Article
- Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.Clinical and experimental reproductive medicine · 2025Article
- A novel approach to determine the critical survival threshold of cellular oxygen within spheroids via integrating live/dead cell imaging with oxygen modeling.American journal of physiology. Cell physiology · 2024Article
- Three-dimensional oxygen concentration monitoring in hydrogels using low-cost phosphorescence lifetime imaging for tissue engineering.Biomedical optics express · 2023Article
- Article
- Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device.Organs-on-a-chip · 2022Article
- Sensor technologies for quality control in engineered tissue manufacturing.Biofabrication · 2022Review
- Computational Modeling and Imaging of the Intracellular Oxygen Gradient.International journal of molecular sciences · 2022Article
- Harnessing conserved signaling and metabolic pathways to enhance the maturation of functional engineered tissues.NPJ Regenerative medicine · 2022Review
- Development of a Versatile, Low-Cost Electrochemical System to Study Biofilm Redox Activity at the Micron Scale.Applied and environmental microbiology · 2022Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Cancer Stem Cells in Tumor Modeling: Challenges and Future Directions.Advanced nanobiomed research · 2021Article
- Optimization of Oxygen Delivery Within Hydrogels.Journal of biomechanical engineering · 2021Review
- Real-Time Analysis of Oxygen Gradient in Oocyte Respiration Using a High-Density Microelectrode Array.Biosensors · 2021Article
- Oxygen Biosensors and Control in 3D Physiomimetic Experimental Models.Antioxidants (Basel, Switzerland) · 2021Review
- In-Line Analysis of Organ-on-Chip Systems with Sensors: Integration, Fabrication, Challenges, and Potential.ACS biomaterials science & engineering · 2021Article
- Microvascular Tissue Engineering-A Review.Biomedicines · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Phase fluorimetry, unlike the more commonly used intensity-based measurement, is not affected by differences in light paths from culture vessels or by optical attenuation through dense 3D cell cultures and hydrogels thereby minimizing dependence on signal intensity for accurate measurements. This work describes the use of phase fluorimetry on oxygen-sensor microbeads to perform oxygen measurements in different microtissue culture environments. In one example, cell spheroids were observed to deplete oxygen from the cell-culture medium filling the bottom of conventional microwells within minutes, whereas oxygen concentrations remained close to ambient levels for several days in hanging-drop cultures. By dispersing multiple oxygen microsensors in cell-laden hydrogels, we also mapped cell-generated oxygen gradients. The spatial oxygen mapping was sufficiently precise to enable the use of computational models of oxygen diffusion and uptake to give estimates of the cellular oxygen uptake rate and the half-saturation constant. The results show the importance of integrated design and analysis of 3D cell cultures from both biomaterial and oxygen supply aspects. While this paper specifically tests spheroids and cell-laden gel cultures, the described methods should be useful for measuring pericellular oxygen concentrations in a variety of biomaterials and culture formats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.