ArticleAnalytical chemistry2017
Probing Membrane Receptor-Ligand Specificity with Surface- and Tip- Enhanced Raman Scattering.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The development and application of SERS-based lateral flow immunochromatography in the field of food safety.Mikrochimica acta · 2025Review
- Surface-Enhanced Raman Spectroscopy at the Interface between Drug Discovery and Personalized Medicine.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Review
- The effects of sun exposure on colorant identification of permanently and semi-permanently dyed hair.Scientific reports · 2023Article
- A Wide-Field Imaging Approach for Simultaneous Super-Resolution Surface-Enhanced Raman Scattering Bioimaging and Spectroscopy.ACS measurement science au · 2022Article
- Super-Resolution SERS Spectral Bioimaging.Proceedings of SPIE--the International Society for Optical Engineering · 2022Article
- Storage Conditions of Human Kidney Tissue Sections Affect Spatial Lipidomics Analysis Reproducibility.Journal of the American Society for Mass Spectrometry · 2020Article
- Super-resolution Surface-Enhanced Raman Scattering Imaging of Single Particles in Cells.Analytical chemistry · 2020Article
- Infrared and Raman chemical imaging and spectroscopy at the nanoscale.Chemical Society reviews · 2020Article
- Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes.Micromachines · 2020Article
- Article
- Label-free plasmonic nanostar probes to illuminateChemical science · 2019Article
- Probing Membrane Receptors with Enhanced Raman Imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2018Article
- Spectroscopic Imaging at the Nanoscale: Technologies and Recent Applications.Analytical chemistry · 2018Review
- SERS-fluorescence bimodal nanoprobes for in vitro imaging of fatty acid responsive receptor GPR120.Analytical methods : advancing methods and applications · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The specific interaction between a ligand and a protein is a key component in minimizing off-target effects in drug discovery. Investigating these interactions with membrane protein receptors can be quite challenging. In this report, we show how spectral variance observed in surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) can be correlated with ligand specificity in affinity-based assays. Variations in the enhanced Raman spectra of three peptide ligands (i.e., cyclic-RGDFC, cyclic-isoDGRFC, and CisoDGRC), which have different binding affinity to αvβ3 integrin, are reported from isolated proteins and from receptors in intact cancer cell membranes. The SERS signal from the purified proteins provides basis spectra to analyze the signals in cells. Differences in the spectral variance within the SERS and TERS data for each ligand indicate larger variance for nonspecific ligand-receptor interactions. The SERS and TERS results are correlated with single particle tracking experiments of the ligand-functionalized nanoparticles with purified receptors on glass surfaces and living cells. These results demonstrate the ability to elucidate protein-ligand recognition using the observed vibrational spectra and provide perspective on binding specificity for small-molecule ligands in intact cell membranes, demonstrating a new approach for investigating drug specificity.
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Registered trials
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