ArticleIranian journal of basic medical sciences2017
A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies.
Article in Iranian journal of basic medical sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Development of monoclonal antibody-based methods for detecting mpox virus.Biosafety and health · 2026Article
- In silico design ofHeliyon · 2024Article
- Antibodies as key mediators of protection againstFrontiers in immunology · 2024Review
- Fatty-Acid-Binding Proteins: From Lipid Transporters to Disease Biomarkers.Biomolecules · 2023Review
- Silicon Nanodisk Huygens Metasurfaces for Portable and Low-Cost Refractive Index and Biomarker Sensing.ACS applied nano materials · 2022Article
- Accuracy of multitarget indirect enzyme-linked immunoassay assay for detection of tuberculosis antibody.Annals of translational medicine · 2021Article
- Surface Enhanced CdSe/ZnS QD/SiNP Electrochemical Immunosensor for the Detection ofMaterials (Basel, Switzerland) · 2019Article
- Application of Laser Scanning Confocal Microscopy for the Visualization ofJournal of clinical medicine · 2019Article
- Enhanced sensitivity of capture IgE‑ELISA based on a recombinant Der f 1/2 fusion protein for the detection of IgE antibodies targeting house dust mite allergens.Molecular medicine reports · 2019Article
- Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode.Sensors (Basel, Switzerland) · 2018Article
- Application of antigenic biomarkers for Mycobacterium tuberculosis.Journal of Zhejiang University. Science. BReview
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10 authors.
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Abstract
objectivesDetection of circulating MATERIALS AND
methodsA fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed. The fusion polyprotein was used as an immunogen to elicit production of multi-target antibodies. Their reactivities were tested. Then, the multi-target antibodies and three corresponding antibodies elicited by each single antigen (mono-target antibodies) were evaluated with sandwich ELISA for detecting
resultsThe polyprotein successfully elicited production of multi-target antibodies targeting 38kD, ESAT6, and CFP10 as analyzed by Western blotting. When used as coating antibodies, the multi-target antibodies were more efficient in capturing the three antigens than the corresponding mono-target antibodies. By testing clinical serum, the multi-target antibodies demonstrated significantly higher sensitivity for clinical TB diagnosis than all three mono-target antibodies.
conclusionThe multi-target antibodies allowed detecting multiple antigens simultaneously and significantly enhanced TB detection compared to routine mono-target antibodies. Our study may provide a promising strategy for TB diagnosis.
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