ArticleInternational journal of legal medicine2018
Rapid screening method for male DNA by using the loop-mediated isothermal amplification assay.
Article in International journal of legal medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- An integrated extraction-free LAMP platform with colorimetric, real-time, and immunochromatographic strip detection for rapid male-specific DNA screening in forensic triage.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Enhancing the forensic sexual assault investigations with LAMP-based male DNA detection.Forensic science international. Synergy · 2025Article
- RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics.ACS synthetic biology · 2024Review
- Duplex loop-mediated isothermal amplification assay for simultaneous detection of human and human male DNA.BMC research notes · 2023Article
- Application of the colorimetric loop-mediated isothermal amplification (LAMP) technique for genotyping Cre-driver mice.The Journal of veterinary medical science · 2022Article
- A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction.Analytica chimica acta · 2019Article
- A LAMP assay for the rapid and robust assessment of Wolbachia infection in Aedes aegypti under field and laboratory conditions.PloS one · 2019Article
- The detection and identification of saliva in forensic samples by RT-LAMP.Forensic science, medicine, and pathology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Screening for male-derived biological material from collected samples plays an important role in criminal investigations, especially those involving sexual assaults. We have developed a loop-mediated isothermal amplification (LAMP) assay targeting multi-repeat sequences of the Y chromosome for detecting male DNA. Successful amplification occurred with 0.5 ng of male DNA under isothermal conditions of 61 to 67 °C, but no amplification occurred with up to 10 ng of female DNA. Under the optimized conditions, the LAMP reaction initiated amplification within 10 min and amplified for 20 min. The LAMP reaction was sensitive at levels as low as 1-pg male DNA, and a quantitative LAMP assay could be developed because of the strong correlation between the reaction time and the amount of template DNA in the range of 10 pg to 10 ng. Furthermore, to apply the LAMP assay to on-site screening for male-derived samples, we evaluated a protocol using a simple DNA extraction method and a colorimetric intercalating dye that allows detection of the LAMP reaction by evaluating the change in color of the solution. Using this protocol, samples of male-derived blood and saliva stains were processed in approximately 30 min from DNA extraction to detection. Because our protocol does not require much hands-on time or special equipment, this LAMP assay promises to become a rapid and simple screening method for male-derived samples in forensic investigations.
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