Evidence map›Paper›PMID 28801881›Full record

ArticleMolecular biotechnology2017

Transgenic Analysis Reveals 5' Abbreviated OsRGLP2 Promoter(s) as Responsive to Abiotic Stresses.

Shahzad Hussain Shah, Azka Noureen, Farah Deeba, Tasawar Sultana, Stefanie Dukowic-Schulze, Changbin Chen, Syed Muhammad Saqlan Naqvi

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Article in Molecular biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Shahzad Hussain ShahDepartment of Biochemistry, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.
Azka NoureenDepartment of Biochemistry, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.
Farah DeebaDepartment of Biochemistry, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.
Tasawar SultanaDepartment of Biochemistry, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.
Stefanie Dukowic-SchulzeDepartment of Horticultural Science, University of Minnesota, St. Paul, MN 55108, USA.
Changbin ChenDepartment of Horticultural Science, University of Minnesota, St. Paul, MN 55108, USA.
Syed Muhammad Saqlan NaqviDepartment of Biochemistry, PMAS- Arid Agriculture University, Rawalpindi, Pakistan. saqlan@uaar.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Germins and germin-like proteins are ubiquitous, expressed at various developmental stages and in response to various abiotic and biotic stresses. In this study, to functionally validate the OsRGLP2 promoter, 5' deletion analysis of the promoter sequences was performed and the deletion fragments fused with the β-glucuronidase (GUS) and green fluorescent protein reporter genes were used for transient expression in tobacco as well as for generating stable transgenic Arabidopsis plants. Very high level of GUS activity was observed in agroinfiltrated tobacco leaves by the construct carrying the P-1063 and P-565 when subjected to abiotic stresses. Histochemical analysis of transgenic Arabidopsis plants revealed expression of reporter gene in root, leaf and stem sections of plants harboring P-1063 and P-565. Real-time qPCR analysis of transiently expressed tobacco leaves and transgenic Arabidopsis plants subjected to several abiotic stresses supported histochemical data and showed that P-565 responded to all the stresses to which the full-length promoter was responsive. The data suggest that P-565 may be a good alternative to full-length promoter region that harbors the necessary cis-elements in providing stable and high level of expression in response to wound, salt and temperature stresses.

Indexed as

Gene Expression Regulation, PlantPromoter Regions, GeneticStress, Physiological5' Flanking RegionArabidopsisDNA, PlantGenes, ReporterNicotianaOryzaPlant ProteinsPlants, Genetically ModifiedDNA, PlantPlant ProteinsAbiotic stressesGateway cloningGermin-like proteinsGUS assayOsRGLP2 promoterReal-time qPCR

Identifiers

PMID28801881

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.