ArticleAnalytical and bioanalytical chemistry2017
Simultaneous measurement of total estradiol and testosterone in human serum by isotope dilution liquid chromatography tandem mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Microvascular endothelial function is blunted in postmenopausal females with frequent hot flushes.Experimental physiology · 2026Article
- Highly specific ID-UHPLC-MS/MS method for analyzing polar and non-polar steroid hormones.Analytical and bioanalytical chemistry · 2026Article
- The testobolome in microbial testosterone metabolism and human health.NPJ biofilms and microbiomes · 2026Review
- Vasomotor symptoms of menopause, sympathetic activity, and blood pressure in postmenopausal females.American journal of physiology. Heart and circulatory physiology · 2025Article
- Determination of Hormonal Growth Promotants in Beef Using Liquid Chromatography-Tandem Mass Spectrometry.Drug testing and analysis · 2025Article
- Article
- Association between visceral adipose tissue and total testosterone among the United States male adults: a cross-sectional study.International journal of impotence research · 2025Article
- Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Total and Free Testosterone in Human Serum and Its Application to Monitoring Biomarker Response of Elite Athletes.Molecules (Basel, Switzerland) · 2024Article
- Positive association between serum bilirubin within the physiological range and serum testosterone levels.BMC endocrine disorders · 2024Article
- Is There a Two-Way Risk between Decreased Testosterone Levels and the Progression and Prognosis of Chronic Kidney Disease? A Cohort Study Based on the National Health and Nutrition Examination Survey Database.The world journal of men's health · 2024Article
- Sleep disturbance and sympathetic neural reactivity in postmenopausal females.American journal of physiology. Heart and circulatory physiology · 2024Article
- Associations between estradiol and hyperuricemia and the mediating effects of TC, TG, and TyG: NHANES 2013-2016.Frontiers in endocrinology · 2024Article
- Association between polyunsaturated fatty acid intake and estradiol levels among U.S. women.Frontiers in nutrition · 2024Article
- Longitudinal analysis of external quality assessment of immunoassay-based steroid hormone measurement indicates potential for improvement in standardization.Frontiers in molecular biosciences · 2024Article
- The association between sex hormones and prevalence of OA in US adults.Frontiers in medicine · 2024Article
- Dietary Acid Load Is Not Associated with Serum Testosterone in Men: Insights from the NHANES.Nutrients · 2023Article
- Article
- Clinical Usefulness of Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Low Serum Testosterone Measurement.Annals of laboratory medicine · 2023Article
- Reproductive Hormone Concentrations and Associated Anatomical Responses: Does Soy Formula Affect Minipuberty in Boys?The Journal of clinical endocrinology and metabolism · 2021Article
- Serum Total Testosterone and Premature Mortality Among Men in the USA.European urology open science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Reliable measurement of total testosterone and estradiol is critical for their use as biomarkers of hormone-related disorders in patient care and translational research. We developed and validated a mass spectrometry method to simultaneously quantify these analytes in human serum without chemical derivatization. Serum is equilibrated with isotopic internal standards and treated with acidic buffer to release hormones from their binding proteins. Lipids are isolated and polar impurities are removed by two serial liquid-liquid extraction steps. Total testosterone and estradiol are measured using liquid chromatography tandem mass spectrometry (LC-MS/MS) in combination of positive and negative electrospray ionization modes. The method shows broad analytical measurement range for both testosterone 0.03-48.5 nM (0.75-1400 ng/dL) and estradiol 11.0-5138 pM (2.99-1400 pg/mL) and excellent agreement with certified reference materials (mean bias less than 2.1% to SRM 971, BCR 576, 577, and 578) and a high order reference method (mean bias 1.25% for testosterone and -0.84% for estradiol). The high accuracy of the method was monitored and certified by CDC Hormone Standardization (HoSt) Program for 2 years with mean bias -0.7% (95% CI -1.6% to 0.2%) for testosterone and 0.1% (95% CI -2.2% to 2.3%) for estradiol. The method precision over a 2-year period for quality control pools at low, medium, and high concentrations was 2.7-2.9% for testosterone and 3.3-5.3% for estradiol. With the consistently excellent accuracy and precision, this method is readily applicable for high-throughput clinical and epidemiological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.