Evidence map›Paper›PMID 28801748›Full record

ArticlePlanta2017

Molecular and phenotypic characterization of transgenic wheat and sorghum events expressing the barley alanine aminotransferase.

Pamela A Peña, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Ismail M Dweikat, Madhavan Soundararajan, Tom Clemente

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
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  4. Role of alanine aminotransferase in crop resilience to climate change: a critical review.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Pamela A PeñaDepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Truyen QuachCenter for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Shirley SatoCenter for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Zhengxiang GeCenter for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Natalya NersesianCenter for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Ismail M DweikatDepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Madhavan SoundararajanDepartment of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Tom ClementeDepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA. tclemente1@unl.edu.
University of Nebraska–Lincoln · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MAIN

conclusionThe expression of a barley alanine aminotransferase gene impacts agronomic outcomes in a C3 crop, wheat. The use of nitrogen-based fertilizers has become one of the major agronomic inputs in crop production systems. Strategies to enhance nitrogen assimilation and flux in planta are being pursued through the introduction of novel genetic alleles. Here an Agrobacterium-mediated approach was employed to introduce the alanine aminotransferase from barley (Hordeum vulgare), HvAlaAT, into wheat (Triticum aestivum) and sorghum (Sorghum bicolor), regulated by either constitutive or root preferred promoter elements. Plants harboring the transgenic HvAlaAT alleles displayed increased alanine aminotransferase (alt) activity. The enhanced alt activity impacted height, tillering and significantly boosted vegetative biomass relative to controls in wheat evaluated under hydroponic conditions, where the phenotypic outcome across these parameters varied relative to time of year study was conducted. Constitutive expression of HvAlaAT translated to elevation in wheat grain yield under field conditions. In sorghum, expression of HvAlaAT enhanced enzymatic activity, but no changes in phenotypic outcomes were observed. Taken together these results suggest that positive agronomic outcomes can be achieved through enhanced alt activity in a C3 crop, wheat. However, the variability observed across experiments under greenhouse conditions implies the phenotypic outcomes imparted by the HvAlaAT allele in wheat may be impacted by environment.

Indexed as

AgrobacteriumAlanine TransaminaseEdible GrainHordeumNitrogenPhenotypePlant ProteinsPlant RootsPlants, Genetically ModifiedPromoter Regions, GeneticSorghumTransgenesTriticumAlanine TransaminaseNitrogenPlant ProteinsAbiotic stressAgrobacterium tumefaciensBiotechnologyNitrogen use efficiency

Identifiers

PMID28801748
OpenAlexW2743755525

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.