Evidence map›Paper›PMID 28801679›Full record

ArticleScientific reports2017

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.

Seon-Ju Yeo, Duong Tuan Bao, Ga-Eun Seo, Cuc Thi Bui, Do Thi Hoang Kim, Nguyen Thi Viet Anh, Trinh Thi Thuy Tien, Nguyen Thi Phuong Linh, Hae-Jin Sohn, Chom-Kyu Chong and 2 more

RetractedOpen access · goldAbstract readRetracted Publication
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Seon-Ju YeoZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Duong Tuan BaoZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Ga-Eun SeoDepartment of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University, Suwon, Republic of Korea.
Cuc Thi BuiZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.ORCID 0000-0002-9422-187X
Do Thi Hoang KimZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Nguyen Thi Viet AnhZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Trinh Thi Thuy TienZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Nguyen Thi Phuong LinhZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea.
Hae-Jin SohnDepartment of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University, Suwon, Republic of Korea.
Chom-Kyu ChongGenBody Inc, No. 206, Biotech Business IC, DanKook University, San-29, Anseo-dong, Dongnam-gu, Cheonan, Republic of Korea.
Ho-Joon ShinDepartment of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University, Suwon, Republic of Korea. hjshin@ajou.ac.kr.
Hyun ParkZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan, 54538, Republic of Korea. hyunpk@wku.ac.kr.
Wonkwang University · KRAjou University · KRDankook University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle-based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

Indexed as

AnimalsAntibodies, MonoclonalAntibodies, ViralDogsEuropiumFluoroimmunoassayHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H7N9 SubtypeLimit of DetectionMadin Darby Canine Kidney CellsMetal NanoparticlesOrthomyxoviridae InfectionsReagent Kits, DiagnosticRecombinant ProteinsSensitivity and SpecificityAntibodies, MonoclonalAntibodies, ViralEuropiumHemagglutinin Glycoproteins, Influenza VirusReagent Kits, DiagnosticRecombinant Proteins

Identifiers

PMID28801679
PMCPMC5554140
OpenAlexW2743827920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.