ArticleScientific reports2017
Both Nuclear Size and DNA Amount Contribute to Midblastula Transition Timing in Xenopus laevis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026Review
- Regulation of zygotic genome activation by the nucleocytoplasmic ratio.Frontiers in cell and developmental biology · 2026Review
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Cell state transitions are decoupled from cell division during early embryo development.Nature cell biology · 2024Article
- The Nuclear-to-Cytoplasmic Ratio: Coupling DNA Content to Cell Size, Cell Cycle, and Biosynthetic Capacity.Annual review of genetics · 2022Review
- Cullin-5 mutants reveal collective sensing of the nucleocytoplasmic ratio in Drosophila embryogenesis.Current biology : CB · 2022Article
- The DNA-to-cytoplasm ratio broadly activates zygotic gene expression in Xenopus.Current biology : CB · 2021Article
- Modeling the role for nuclear import dynamics in the early embryonic cell cycle.Biophysical journal · 2021Article
- The nuclear to cytoplasmic ratio directly regulates zygotic transcription inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- DNA content contributes to nuclear size control inMolecular biology of the cell · 2020Article
- Organelle size scaling over embryonic development.Wiley interdisciplinary reviews. Developmental biology · 2020Review
- Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo.Developmental cell · 2019Article
- From single cells to tissue self-organization.The FEBS journal · 2019Review
- Mechanisms regulating zygotic genome activation.Nature reviews. Genetics · 2019Review
- Altering the levels of nuclear import factors in early Xenopus laevis embryos affects later development.PloS one · 2019Article
- Subcellular scaling: does size matter for cell division?Current opinion in cell biology · 2018Review
- ELUCIDATING NUCLEAR SIZE CONTROL IN THE XENOPUS MODEL SYSTEM.Veterinarski glasnik · 2018Article
Corrections and comments
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During early Xenopus laevis embryogenesis both nuclear and cell volumes decrease with the nuclear-to-cytoplasmic (N/C) volume ratio reaching a maximum at the midblastula transition (MBT). At the MBT, embryonic transcription is upregulated and cell cycles lengthen. Early studies demonstrated a role for the DNA-to-cytoplasmic ratio in the control of MBT timing. By altering nuclear size, we previously showed that the N/C volume ratio also contributes to proper MBT timing. Here we examine the relative contributions of nuclear size and DNA amount to MBT timing by simultaneously altering nuclear size and ploidy in X. laevis embryos. Compared to diploid embryos, haploids exhibited a delay in both zygotic gene expression and cell cycle lengthening, while diploid embryos with increased N/C volume ratios showed early expression of zygotic genes and premature lengthening of cell cycles. Interestingly, haploids with increased N/C volume ratios exhibited an intermediate effect on the timing of zygotic gene expression and cell cycle lengthening. Decreasing nuclear size in post-MBT haploid embryos caused a further delay in cell cycle lengthening and the expression of some zygotic genes. Our data suggest that both the N/C volume ratio and DNA amount contribute to the regulation of MBT timing with neither parameter being dominant.
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