ArticleScientific reports2017
Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Advances in breeding, biotechnology, and nanotechnological approaches to combat sheath blight disease in rice.Molecular biology reports · 2024Review
- Gene expression of near-isogenic lines (NILs) carrying blast resistance genes Pi9 and Pi54 in the background of rice cultivar Mushk Budji.Molecular biology reports · 2023Article
- Novel Sulfonamide Derivatives Containing a Piperidine Moiety as New Bactericide Leads for Managing Plant Bacterial Diseases.International journal of molecular sciences · 2023Article
- Suppressing chlorophyll degradation by silencing OsNYC3 improves rice resistance to Rhizoctonia solani, the causal agent of sheath blight.Plant biotechnology journal · 2022Article
- Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani.Rice (New York, N.Y.) · 2021Review
- Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Understanding sheath blight resistance in rice: the road behind and the road ahead.Plant biotechnology journal · 2020Review
- Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses againstInternational journal of environmental research and public health · 2020Article
- Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation.Molecular biotechnology · 2020Article
- Transgene Stacking as Effective Tool for Enhanced Disease Resistance in Plants.Molecular biotechnology · 2020Review
- Genome-Wide Association Analysis of the Genetic Basis for Sheath Blight Resistance in Rice.Rice (New York, N.Y.) · 2019Article
- Review
- Biosynthesis of silver nanoparticles using endophytic bacteria and their role in inhibition of rice pathogenic bacteria and plant growth promotion.RSC advances · 2019Article
- Proteo-metabolomic investigation of transgenic rice unravels metabolic alterations and accumulation of novel proteins potentially involved in defence against Rhizoctonia solani.Scientific reports · 2019Article
- Inhibition of OsSWEET11 function in mesophyll cells improves resistance of rice to sheath blight disease.Molecular plant pathology · 2018Article
- The Development ofFrontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of transgenic rice lines. The first transgenic line overexpresses the rice chitinase gene (OsCHI11); the second contains the Arabidopsis NPR1 (AtNPR1) gene and, the third has pyramided constructs with both the genes (OsCHI11 and AtNPR1). This is a comparative study between the single-gene transgenic lines and the double gene transgenic in terms of their ability to activate the plant defense system. Rice plants of each individual construct were screened via PCR, Southern hybridization, activity assays, and expression analysis. The best transgenic lines of each construct were chosen for comparative study. The fold change in qRT-PCR and activity assays revealed that the pyramided transgenic rice plants show a significant upregulation of defense-related genes, PR genes, and antioxidant marker genes as compared to the single transgene. Simultaneous co-expression of both the genes was found to be more efficient in tolerating oxidative stress. In R. solani (RS) toxin assay, mycelial agar disc bioassay, and in vivo plant bioassay, pyramided transgenic plant lines were more competent at restricting the pathogen development and enhancing sheath blight tolerance as compared to single gene transformants.
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