ArticleMalaria journal2017
Life-span of in vitro differentiated Plasmodium falciparum gametocytes.
Article in Malaria journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- The inducible gametocyte producer (iGP1) strain is well-suited to produce both immature and mature gametocytes for subsequent drug sensitivity profiling.Frontiers in cellular and infection microbiology · 2026Article
- Biology and epidemiology ofParasite epidemiology and control · 2023Review
- Direct injection of Amblyomma americanum ticks with Cytauxzoon felis.Ticks and tick-borne diseases · 2022Article
- Gametocyte carriage of Plasmodium falciparum (pfs25) and Plasmodium vivax (pvs25) during mass screening and treatment in West Timor, Indonesia: a longitudinal prospective study.Malaria journal · 2021Article
- Plasmodium-a brief introduction to the parasites causing human malaria and their basic biology.Journal of physiological anthropology · 2021Review
- Viability and Infectivity ofFrontiers in cellular and infection microbiology · 2021Article
- Novel Method for the Separation of Male and Female Gametocytes of the Malaria ParasitemSphere · 2020Article
- Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2).Malaria journal · 2020Article
- Modeling the dynamics ofeLife · 2019Article
- Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies.EBioMedicine · 2019Article
- Characterization of Plasmodium infections among inhabitants of rural areas in Gabon.Scientific reports · 2019Article
- Gametocyte Development and Carriage in Ghanaian Individuals with UncomplicatedThe American journal of tropical medicine and hygiene · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sexual stages (gametocytes) of Plasmodium falciparum do not directly contribute to the pathology of malaria but are essential for transmission of the parasite from the human host to the mosquito. Mature gametocytes circulate in infected human blood for several days and their circulation time has been modelled mathematically from data of previous in vivo studies. This is the first time that longevity of gametocytes is studied experimentally in vitro.
methodsThe in vitro longevity of P. falciparum gametocytes of 1 clinical isolate and 2 laboratory strains was assessed by three different methods: microscopy, flow cytometry and reverse transcription quantitative real-time PCR (RT-qPCR). Additionally, the rate of gametocytogenesis of the used P. falciparum strains was compared.
resultsThe maximum in vitro lifespan of P. falciparum gametocytes reached almost 2 months (49 days by flow cytometry, 46 days by microscopy, and at least 52 days by RT-qPCR) from the starting day of gametocyte culture to death of last parasite in the tested strains with an average 50% survival rate of 6.5, 2.6 and 3.5 days, respectively. Peak gametocytaemia was observed on average 19 days after initiation of gametocyte culture followed by a steady decline due to natural decay of the parasites. The rate of gametocytogenesis was highest in the NF54 strain.
conclusionsPlasmodium falciparum mature gametocytes can survive up to 16-32 days (at least 14 days for mature male gametocytes) in vitro in absence of the influence of host factors. This confirms experimentally a previous modelling estimate that used molecular tools for gametocyte detection in treated patients. The survival time might reflect the time the parasite can be transmitted to the mosquito after clearance of asexual parasites. These results underline the importance of efficient transmission blocking agents in the fight against malaria.
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