ArticlePloS one2017
Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
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- Mind your tag in single-molecule measurements.Cell reports methods · 2023Article
- Speg interactions that regulate the stability of excitation-contraction coupling protein complexes in triads and dyads.Communications biology · 2023Article
- Protein fluorescent labeling in live yeast cells using scFv-based probes.Cell reports methods · 2022Article
- A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.G3 (Bethesda, Md.) · 2022Article
- Breasi-CRISPR: an efficient genome-editing method to interrogate protein localization and protein-protein interactions in the embryonic mouse cortex.Development (Cambridge, England) · 2022Article
- Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26.The FEBS journal · 2022Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Protocol for using fluorescent sensors targeted to endogenous proteins (FluoSTEPs) to measure microdomain-specific signaling events.STAR protocols · 2021Article
- Evaluation of the OsTIR1 and AtAFB2 AID Systems for Genome Architectural Protein Degradation in Mammalian Cells.Frontiers in molecular biosciences · 2021Article
- Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits.The Plant cell · 2020Article
- Bi-allelic LoF NRROS Variants Impairing Active TGF-β1 Delivery Cause a Severe Infantile-Onset Neurodegenerative Condition with Intracranial Calcification.American journal of human genetics · 2020Article
- Glycine-alanine dipeptide repeats spread rapidly in a repeat length- and age-dependent manner in the fly brain.Acta neuropathologica communications · 2019Article
- Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in theG3 (Bethesda, Md.) · 2019Article
- PP2C phosphatases promote autophagy by dephosphorylation of the Atg1 complex.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle.PloS one · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different systems have been developed during years in the yeast Saccharomyces cerevisiae. In the present study, we analysed systematically a set of yeast proteins that were fused to different tags. Analysis of the tagged proteins revealed an unexpected general effect on protein level when some specific tagging module was used. This was due in all cases to a destabilization of the proteins and caused a reduced protein activity in the cell that was only apparent in particular conditions. Therefore, an extremely cautious approach is required when using this strategy.
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