ArticlePloS one2017
Species identification and molecular typing of human Brucella isolates from Kuwait.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Evaluation of AI-enhanced FTIR spectroscopy for species and biovar typing of intracellular pathogenic Brucella spp.BMC microbiology · 2026Article
- Whole-Genome Sequencing of Brucella melitensis Isolates from Kuwait for the Identification of Biovars, Variants, and Relationship within a Biovar.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2025Article
- Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses.Veterinary research communications · 2024Review
- Long ignored but making a comeback: a worldwide epidemiological evolution of human brucellosis.Emerging microbes & infections · 2024Review
- Whole Genome Sequencing: Applications in Clinical Bacteriology.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2024Review
- Antibiotic Resistance Mediated byAntibiotics (Basel, Switzerland) · 2023Article
- Genome survey and genetic characterization ofFrontiers in plant science · 2023Article
- Composition of nasal bacterial community and its seasonal variation in health care workers stationed in a clinical research laboratory.PloS one · 2021Article
- Sheep Brucellosis in Kuwait: A Large-Scale Serosurvey, Identification ofVeterinary sciences · 2020Article
- Retracted: Optimization of Precontrol Methods and Analysis of a Dynamic Model for Brucellosis: Model Development and Validation.JMIR medical informatics · 2020Article
- Pediatric brucellosis. An update review for the new millennium.Saudi medical journal · 2018Review
- Multiple Locus Variable-Number Tandem-Repeat and Single-Nucleotide Polymorphism-BasedFrontiers in veterinary science · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucellosis is a zoonotic disease of major concern in Kuwait and the Middle East. Human brucellosis can be caused by several Brucella species with varying degree of pathogenesis, and relapses are common after apparently successful therapy. The classical biochemical methods for identification of Brucella are time-consuming, cumbersome, and provide information limited to the species level only. In contrast, molecular methods are rapid and provide differentiation at intra-species level. In this study, four molecular methods [16S rRNA gene sequencing, real-time PCR, enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus variable-number tandem-repeat analysis (MLVA)-8, MLVA-11 and MLVA-16 were evaluated for the identification and typing of 75 strains of Brucella isolated in Kuwait. 16S rRNA gene sequencing of all isolates showed 90-99% sequence identity with B. melitensis and real-time PCR with genus- and species- specific primers identified all isolates as B. melitensis. The results of ERIC-PCR suggested the existence of 75 ERIC genotypes of B. melitensis with a discriminatory index of 0.997. Cluster classification of these genotypes divided them into two clusters, A and B, diverging at ~25%. The maximum number of genotypes (n = 51) were found in cluster B5. MLVA-8 analysis identified all isolates as B. melitensis, and MLVA-8, MLVA-11 and MLVA-16 typing divided the isolates into 10, 32 and 71 MLVA types, respectively. Furthermore, the combined minimum spanning tree analysis demonstrated that, compared to MLVA types discovered all over the world, the Kuwaiti isolates were a distinct group of MLVA-11 and MLVA-16 types in the East Mediterranean Region.
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