ArticleNanomaterials (Basel, Switzerland)2017
Response of Dermal Fibroblasts to Biochemical and Physical Cues in Aligned Polycaprolactone/Silk Fibroin Nanofiber Scaffolds for Application in Tendon Tissue Engineering.
Article in Nanomaterials (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 69 citations in OpenAlex.
- Composite Polycaprolactone/Gelatin Nanofiber Membrane Scaffolds for Mesothelial Cell Culture and Delivery in Mesothelium Repair.International journal of molecular sciences · 2024Article
- Advances in the application of hydrogel-based scaffolds for tendon repair.Genes & diseases · 2024Review
- Bioactive Nanostructured Scaffold-Based Approach for Tendon and Ligament Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2023Review
- A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering.Polymers · 2022Review
- Advanced Nanofiber-Based Scaffolds for Achilles Tendon Regenerative Engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Tension Stimulation of Tenocytes in Aligned Hyaluronic Acid/Platelet-Rich Plasma-Polycaprolactone Core-Sheath Nanofiber Membrane Scaffold for Tendon Tissue Engineering.International journal of molecular sciences · 2021Article
- The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research.Frontiers in cell and developmental biology · 2021Review
- Review
- From the perspective of embryonic tendon development: various cells applied to tendon tissue engineering.Annals of translational medicine · 2020Review
- Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon Stem/Progenitor Cells.Applied bionics and biomechanics · 2020Article
- Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds.Nanomaterials (Basel, Switzerland) · 2019Article
- Histological evaluation of tendon formation using a scaffold-free three-dimensional-bioprinted construct of human dermal fibroblasts underRegenerative therapy · 2019Article
- Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts.Nanomaterials (Basel, Switzerland) · 2019Article
- Nanofibers as Bioinstructive Scaffolds Capable of Modulating Differentiation through Mechanosensitive Pathways for Regenerative Engineering.Regenerative engineering and translational medicine · 2019Article
- Core-Shell Nanofibrous Scaffold Based on Polycaprolactone-Silk Fibroin Emulsion Electrospinning for Tissue Engineering Applications.Bioengineering (Basel, Switzerland) · 2018Article
- Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges.Materials (Basel, Switzerland) · 2018Review
- High-Throughput Preparation of Silk Fibroin Nanofibers by Modified Bubble-Electrospinning.Nanomaterials (Basel, Switzerland) · 2018Article
- Early cessation of pressure garment therapy results in scar contraction and thickening.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications. The physicochemical properties of random PCL (RP) nanofibers, random PCL/SF (RPSF) nanofibers and aligned PCL/SF (APSF) nanofibers were characterized for fiber orientation and SF blending effects. An in vitro cell culture with rabbit dermal fibroblasts (RDFBs) on nanofibers indicated that SF promotes cell proliferation to a higher extent than fiber alignment. Cells aligned in the direction of fiber axes could be confirmed through scanning electron microscopy (SEM) observation and cytoskeleton staining. The quantitative real-time polymerase chain reaction (qRT-PCR) experiments indicated up-regulated gene expression of tendon marker proteins (type I collagen (Col I), fibronectin and biglycan) on APSF nanofibers and tendon reconstruction was confirmed from Col III gene expression. Animal experiments with Achilles tendon defect repairs in rabbits were carried out with RPSF and APSF scaffolds. The beneficial effects of fiber alignment were verified from histological and immunohistochemical staining, where cell migration and extracellular matrix protein deposition tend to stretch in a parallel direction along the axial direction of APSF nanofibers with enhanced Col I and tenascin C production. Biomechanical testing indicated the tensile stiffness and maximum load of cell-seeded APSF scaffolds were 60.2 and 81.3% of normal tendon values, respectively, which are significantly higher than cell-seeded RPSF or acellular APSF and RPSF scaffolds. These results suggest that APSF nanofiber scaffolds combined with RDFBs have the potential to repair the gap defects of Achilles tendons in vivo and to effectively restore the function and structure of tendons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.