ArticleInternational journal of molecular sciences2017
Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- The potential role of SCF combined with DPCs in facial nerve repair.Journal of molecular histology · 2025Article
- Review
- The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp.International journal of dentistry · 2024Article
- From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction.International journal of molecular sciences · 2022Review
- Therapeutic potential of dental pulp stem cells and their derivatives: Insights from basic research toward clinical applications.World journal of stem cells · 2022Review
- Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism.Biomedicines · 2022Article
- Neural Crest-Derived Stem Cells (NCSCs) Obtained from Dental-Related Stem Cells (DRSCs): A Literature Review on Current Knowledge and Directions toward Translational Applications.International journal of molecular sciences · 2022Review
- Complement C5aR/LPS-induced BDNF and NGF modulation in human dental pulp stem cells.Scientific reports · 2022Article
- Application of dental pulp stem cells in oral maxillofacial tissue engineering.International journal of medical sciences · 2022Review
- Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice.Journal of applied oral science : revista FOB · 2021Article
- Enzyme-digestedNeural regeneration research · 2020Article
- Article
- Review
- The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells.International journal of molecular sciences · 2020Article
- Current advances in ischemic stroke research and therapies.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- Characteristics and Therapeutic Potential of Dental Pulp Stem Cells on Neurodegenerative Diseases.Frontiers in neuroscience · 2020Review
- Biological Behavioral Alterations of the Post-neural Differentiated Dental Pulp Stem Cells Through anFrontiers in cell and developmental biology · 2020Article
- Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice.International journal of molecular sciences · 2019Article
- Dental Pulp Stem Cells: An Attractive Alternative for Cell Therapy in Ischemic Stroke.Frontiers in neurology · 2019Review
- Mechanisms underlying dental-derived stem cell-mediated neurorestoration in neurodegenerative disorders.Stem cell research & therapy · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adult mammalian central nerve system has fundamental difficulties regarding effective neuroregeneration. The aim of this study is to investigate whether human dental pulp cells (DPCs) can promote neuroregeneration by (i) being differentiated toward neuronal cells and/or (ii) stimulating local neurogenesis in the adult hippocampus. Using immunostaining, we demonstrated that adult human dental pulp contains multipotent DPCs, including STRO-1, CD146 and P75-positive stem cells. DPC-formed spheroids were able to differentiate into neuronal, vascular, osteogenic and cartilaginous lineages under osteogenic induction. However, under neuronal inductive conditions, cells in the DPC-formed spheroids differentiated toward neuronal rather than other lineages. Electrophysiological study showed that these cells consistently exhibit the capacity to produce action potentials, suggesting that they have a functional feature in neuronal cells. We further co-cultivated DPCs with adult mouse hippocampal slices on matrigel in vitro. Immunostaining and presto blue assay showed that DPCs were able to stimulate the growth of neuronal cells (especially neurons) in both the CA1 zone and the edges of the hippocampal slices. Brain-derived neurotrophic factor (BDNF), was expressed in co-cultivated DPCs. In conclusion, our data demonstrated that DPCs are well-suited to differentiate into the neuronal lineage. They are able to stimulate neurogenesis in the adult mouse hippocampus through neurotrophic support in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.