Evidence map›Paper›PMID 28799649›Full record

ArticleBiopolymers2017

Design of modular dual enzyme-responsive peptides.

Natalie Boehnke, Heather D Maynard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biopolymers, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Natalie BoehnkeDepartment of Chemistry and Biochemistry and California NanoSystems Institute, University of California, 607 Charles E. Young Drive East, Los Angeles, California, 90095.
Heather D MaynardDepartment of Chemistry and Biochemistry and California NanoSystems Institute, University of California, 607 Charles E. Young Drive East, Los Angeles, California, 90095.ORCID http://orcid.org/0000-0003-3692-6289

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
UC BRAID Center for Accelerated Innovation COVID-19 diagnostic project developmentU54HL119893 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PALAZZOLO, MICHAEL J · 2013 to 2020
$18.3M
NCATS NIH HHS UL1 TR000124NHLBI NIH HHS U54 HL119893
6 · The paper itself

Abstract

Dual enzyme-responsive peptides were synthesized by masking the ɛ-amine of lysine with various enzyme substrates. Enzymatic cleavage of these sequences unmasked the ɛ-amine, allowing for further digestion by a second enzyme, which was monitored colorimetrically. This modular peptide design should provide substrates for a large combination of clinically relevant enzymes.

Indexed as

Amino Acid SequenceCaspase 3ChymotrypsinEnzymesKineticsLysinePeptidesSubstrate SpecificityTrypsinCaspase 3ChymotrypsinEnzymesLysinePeptidesTrypsindual-enzymedual-enzyme assayindirect enzyme detectionmulti-enzymemulti-enzyme assaynitroanilide

Identifiers

PMID28799649
PMCPMC5963286

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.