ArticleBiomicrofluidics2017
A microfluidic device for isolation and characterization of transendothelial migrating cancer cells.
Article in Biomicrofluidics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Microfluidic Applications in Prostate Cancer Research.Micromachines · 2024Review
- Insights into the mechanobiology of cancer metastasis via microfluidic technologies.APL bioengineering · 2024Review
- Unraveling Cancer Metastatic Cascade Using Microfluidics-based Technologies.Biophysical reviews · 2022Review
- Application of Microfluidic Systems for Breast Cancer Research.Micromachines · 2022Review
- Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response.Communications biology · 2021Article
- Integrative microphysiological tissue systems of cancer metastasis to the liver.Seminars in cancer biology · 2021Review
- Nanotechnology-Based Strategies to Evaluate and Counteract Cancer Metastasis and Neoangiogenesis.Advanced healthcare materials · 2021Review
- Visualizing cancer extravasation: from mechanistic studies to drug development.Cancer metastasis reviews · 2021Review
- Biomimetic Microfluidic Platforms for the Assessment of Breast Cancer Metastasis.Frontiers in bioengineering and biotechnology · 2021Review
- The Use of Microfluidic Platforms to Probe the Mechanism of Cancer Cell Extravasation.Advanced healthcare materials · 2020Review
- Review
- Live imaging of Aiptasia larvae, a model system for coral and anemone bleaching, using a simple microfluidic device.Scientific reports · 2019Article
- Microfluidics for studying metastatic patterns of lung cancer.Journal of nanobiotechnology · 2019Review
- Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods.ACS biomaterials science & engineering · 2019Article
- Separation and Characterization of Prostate Cancer Cell Subtype according to Their Motility Using a Multi-Layer CiGiP Culture.Micromachines · 2018Article
- A Microfluidic System for the Investigation of Tumor Cell Extravasation.Bioengineering (Basel, Switzerland) · 2018Article
- Fluid shear stress and tumor metastasis.American journal of cancer research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transendothelial migration of cancer cells is a critical stage in cancer, including breast cancer, as the migrating cells are generally believed to be highly metastatic. However, it is still challenging for many existing platforms to achieve a fully covering endothelium and to ensure transendothelial migration capability of the extracted cancer cells for analyses with high specificity. Here, we report a microfluidic device containing multiple independent cell collection microchambers underneath an embedded endothelium such that the transendothelial-migrated cells can be selectively collected from only the microchambers with full coverage of an endothelial layer. In this work, we first optimize the pore size of a microfabricated supporting membrane for the endothelium formation. We quantify transendothelial migration rates of a malignant human breast cell type (MDA-MB-231) under different shear stress levels. We investigate characteristics of the migrating cells including morphology, cytoskeletal structures, and migration (speed and persistence). Further implementation of this endothelium-embedded microfluidic device can provide important insights into migration and intracellular characteristics related to cancer metastasis and strategies for effective cancer therapy.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.