ArticleScientific reports2017
Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- miRNAs as biomarkers for early cancer detection and their application in the development of new diagnostic tools.Biomedical engineering online · 2021Pooled it
- Comparative analysis of wastewater sample processing methods for antimicrobial resistance surveillance.Microbiology spectrum · 2026Article
- Wide-spectrum profiling of plasma cell-free RNA and the potential for health-monitoring.RNA biology · 2025Article
- miRNA Library Preparation Optimisation for Low-Concentration and Low-Volume Paediatric Plasma Samples.Non-coding RNA · 2025Article
- Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis.Animals : an open access journal from MDPI · 2025Article
- The Role of Extracellular Vesicles and Microparticles in Central Nervous System Disorders: Mechanisms, Biomarkers, and Therapeutic Potential.Cellular and molecular neurobiology · 2024Review
- Circulating Serum Micro-RNA as Non-Invasive Diagnostic Biomarkers of Endometriosis.Biomedicines · 2024Article
- Guidelines for mitochondrial RNA analysis.Molecular therapy. Nucleic acids · 2024Review
- Differential effects of winter cold stress on soil bacterial communities, metabolites, and physicochemical properties in two varieties ofMicrobiology spectrum · 2024Article
- Temporal nanofluid environments induce prebiotic condensation in water.Communications chemistry · 2023Article
- Circulating microRNAs as Reliable Tumor Biomarkers: Opportunities and Challenges Facing Clinical Application.The Journal of pharmacology and experimental therapeutics · 2023Review
- Plasma microrna quantification protocol.Vessel plus · 2023Article
- A Quality by Design Approach in Pharmaceutical Development of Non-Viral Vectors with a Focus on miRNA.Pharmaceutics · 2022Review
- Zinc supplementation modifies brain tissue transcriptome of Apis mellifera honeybees.BMC genomics · 2022Article
- Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR.Genes · 2022Review
- Review
- Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22-5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication.International journal of molecular sciences · 2021Article
- Evaluation of five column-based isolation kits and their ability to extract miRNA from human milk.Journal of cellular and molecular medicine · 2021Article
- Circulating MicroRNAs in Gastrointestinal Cancer.Cancers · 2021Review
- Levels and Characteristics of mRNAs in Spores of Firmicute Species.Journal of bacteriology · 2021Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.