ArticleScientific reports2017
Directional cell expansion requires NIMA-related kinase 6 (NEK6)-mediated cortical microtubule destabilization.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Primary nitrate responses mediated by calcium signalling and diverse protein phosphorylation.Journal of experimental botany · 2020Pooled it
- NEK Family Kinases: Structure, Function, and Role in Disease.Biomolecules · 2025Review
- Involvement of a NIMA-related kinase in cell division of the liverwort Marchantia polymorpha.Plant & cell physiology · 2025Article
- Regulation of cytoskeleton dynamics and its interplay with force in plant cells.Biophysics reviews · 2024Review
- EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.Current biology : CB · 2022Article
- The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.The Plant cell · 2022Review
- Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis.Plants (Basel, Switzerland) · 2021Article
- Phosphorylation of Plant Microtubule-Associated Proteins During Cell Division.Frontiers in plant science · 2019Review
- A Cycloheximide-Sensitive Step in Transverse Microtubule Array Patterning.Plant physiology · 2018Article
- The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus Ends.Current biology : CB · 2018Article
- Update: Plant Cortical Microtubule Arrays.Plant physiology · 2018Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cortical microtubules align perpendicular to the growth axis to determine the direction of cell growth. However, it remains unclear how plant cells form well-organized cortical microtubule arrays in the absence of a centrosome. In this study, we investigated the functions of Arabidopsis NIMA-related kinase 6 (NEK6), which regulates microtubule organization during anisotropic cell expansion. Quantitative analysis of hypocotyl cell growth in the nek6-1 mutant demonstrated that NEK6 suppresses ectopic outgrowth and promotes cell elongation in different regions of the hypocotyl. Loss of NEK6 function led to excessive microtubule waving and distortion, implying that NEK6 suppresses the aberrant cortical microtubules. Live cell imaging showed that NEK6 localizes to the microtubule lattice and to the shrinking plus and minus ends of microtubules. In agreement with this observation, the induced overexpression of NEK6 reduced and disorganized cortical microtubules and suppressed cell elongation. Furthermore, we identified five phosphorylation sites in β-tubulin that serve as substrates for NEK6 in vitro. Alanine substitution of the phosphorylation site Thr166 promoted incorporation of mutant β-tubulin into microtubules. Taken together, these results suggest that NEK6 promotes directional cell growth through phosphorylation of β-tubulin and the resulting destabilization of cortical microtubules.
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