ArticlePloS one2017
Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- The effect of bioactive membrane in alveolar ridge preservation: A double-blind randomized clinical trial.Journal of periodontology · 2026Trial
- In vitro and in vivo assessment of a new acellular human amnion/chorion membrane device for guided bone regeneration.Scientific reports · 2025Article
- Clinical trial landscape for guided bone regeneration: trend analysis and future perspectives.Frontiers in dental medicine · 2025Article
- Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells.Bone reports · 2024Article
- The Categorization of Perinatal Derivatives for Orthopedic Applications.Biomedicines · 2024Review
- Preparation of human amniotic membrane for transplantation in different application areas.Frontiers in transplantation · 2023Review
- Epidermal growth factor signalling pathway in endochondral ossification: an evidence-based narrative review.Annals of medicine · 2022Review
- Osteogenic Efficacy of Human Trophoblasts-Derived Conditioned Medium on Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Article
- Fabrication of Decellularized Amnion and Chorion Scaffolds to Develop Bioengineered Cell-Laden Constructs.Cellular and molecular bioengineering · 2022Article
- Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy.Cells · 2022Article
- Comprehensive analysis of human chorionic membrane extracts regulating mesenchymal stem cells during osteogenesis.Cell proliferation · 2022Article
- Amnion and chorion matrix maintain hMSC osteogenic response and enhance immunomodulatory and angiogenic potential in a mineralized collagen scaffold.Frontiers in bioengineering and biotechnology · 2022Article
- Osteogenic effects of exosomes derived from human chorion membrane extracts.Biomaterials research · 2021Article
- Effects of Amniotic Membrane Extract on the Hyperplastic Response of the Middle Ear Mucosa in a Bacterially-Induced Otitis Media Rat Model: A Preliminary Study.Clinical and experimental otorhinolaryngology · 2020Article
- Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions.Regenerative biomaterials · 2020Article
- A Review on Modifications of Amniotic Membrane for Biomedical Applications.Frontiers in bioengineering and biotechnology · 2020Review
- A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy.Eye and vision (London, England) · 2019Article
- Human amniotic membrane for guided bone regeneration of calvarial defects in mice.Journal of materials science. Materials in medicine · 2018Article
- Experimental study of tendon sheath repair via decellularized amnion to prevent tendon adhesion.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic differentiation of osteoblast-like MG-63 cells. MG-63 cells were cultured in osteogenic induction medium (OIM) with or without exogenous AME and CME. CME enhanced the osteogenic differentiation of MG-63 cells compared with AME, as indicated by increased mineralization; alkaline phosphatase activity; and mRNA expression of osteogenic marker genes encoding integrin-binding sialoprotein (IBSP), RUNX2, OSTERIX, and osteocalcin (OCN). Interestingly, AME and CME contained different combinations of osteogenesis-related growth factors, including basic fibroblast growth factor (bFGF), transforming growth factor beta-1 (TGFβ-1), and epidermal growth factor (EGF), which differentially regulated the osteogenic differentiation of MG-63 cells. bFGF and TGFβ-1 present in CME positively regulated the osteogenic differentiation of MG-63 cells, whereas EGF present in AME negatively regulated the differentiation of MG-63 cells. Moreover, exogenous treatment of EGF antagonized CME-induced mineralization of extracellular matrix on MG-63 cells. We compared the osteogenic efficacy of CME with that of BMP2, bFGF, and TGFβ-1 alone or their combinations. We observed that CME greatly enhanced osteogenesis by providing a conductive environment for the differentiation of MG-63 cells. Together, our results indicated that human AME and CME exerted differential effects on osteogenesis because of the presence of different compositions of growth factors. In addition, our results highlighted a new possible strategy of using CME as a biocompatible therapeutic material for bone regeneration.
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