ArticlePlant biotechnology journal2018
Nanobody-mediated resistance to Grapevine fanleaf virus in plants.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Nanobodies: a new frontier in plant disease management.Trends in biotechnology · 2026Review
- Occurrence of Grapevine fanleaf virus (Nepovirus foliumflabelli) in the Western Black Sea region of Türkiye and phylogenetic analysis based on partial coat protein gene.Scientific reports · 2025Article
- Emerging technologies for in-field plant virus detection: innovations and future directions.The Journal of general virology · 2025Review
- Next-Generation Therapeutic Antibodies for Cancer Treatment: Advancements, Applications, and Challenges.Molecular biotechnology · 2025Review
- Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector.Molecular plant pathology · 2025Article
- Development and Applications of Somatic Embryogenesis in Grapevine (Plants (Basel, Switzerland) · 2024Review
- Transient expression of anti-HrpE scFv antibody reduces the hypersensitive response in non-host plant against bacterial phytopathogen Xanthomonas citri subsp. citri.Scientific reports · 2024Article
- NANOBODYInternational journal of molecular sciences · 2023Review
- Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants.Communications biology · 2023Article
- Article
- Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity againstMolecules (Basel, Switzerland) · 2022Article
- Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b.Journal of pharmaceutical analysis · 2022Article
- Transient expression of an scFvG8 antibody in plants and characterization of its effects on the virulence factor pthA of Xanthomonas citri subsp. citri.Transgenic research · 2022Article
- Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective.Plants (Basel, Switzerland) · 2022Review
- Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants.Frontiers in bioengineering and biotechnology · 2022Article
- Development of Nanobodies against Mal de Río Cuarto virus major viroplasm protein P9-1 for diagnostic sandwich ELISA and immunodetection.Scientific reports · 2021Article
- A single resistance factor to solve vineyard degeneration due to grapevine fanleaf virus.Communications biology · 2021Article
- A guide to: generation and design of nanobodies.The FEBS journal · 2021Review
- Applications of nanobodies in plant science and biotechnology.Plant molecular biology · 2021Review
- Exploring cellular biochemistry with nanobodies.The Journal of biological chemistry · 2020Review
Corrections and comments
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution and huge burden on crop yields representing billions of US dollars of losses annually, yet solutions to combat these viruses are often limited or inefficient. Here, we identified a Nb specific to GFLV that confers strong resistance to GFLV upon stable expression in the model plant Nicotiana benthamiana and also in grapevine rootstock, the natural host of the virus. We showed that resistance was effective against a broad range of GFLV isolates independently of the inoculation method including upon nematode transmission but not against its close relative, Arabis mosaic virus. We also demonstrated that virus neutralization occurs at an early step of the virus life cycle, prior to cell-to-cell movement. Our findings will not only be instrumental to confer resistance to GFLV in grapevine, but more generally they pave the way for the generation of novel antiviral strategies in plants based on Nbs.
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Registered trials
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