ArticlePloS one2017
The species origin of the serum in the culture medium influences the in vitro toxicity of silica nanoparticles to HepG2 cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Interaction of Iron Oxide Nanoparticles with Macrophages Is Influenced Distinctly by "Self" and "Non-Self" Biological Identities.ACS applied materials & interfaces · 2023Article
- Defining the influence of size-exclusion chromatography fraction window and ultrafiltration column choice on extracellular vesicle recovery in a skeletal muscle model.Journal of extracellular biology · 2023Article
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins.Advanced healthcare materials · 2021Article
- Environmental dimensions of the protein corona.Nature nanotechnology · 2021Review
- Long-term evolution of the epithelial cell secretome in preclinical 3D models of the human bronchial epithelium.Scientific reports · 2021Article
- Effect of different silica coatings on the toxicity of upconversion nanoparticles on RAW 264.7 macrophage cells.Beilstein journal of nanotechnology · 2021Article
- Insights into Characterization Methods and Biomedical Applications of Nanoparticle-Protein Corona.Materials (Basel, Switzerland) · 2020Review
- Machine learning predicts the functional composition of the protein corona and the cellular recognition of nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine.Beilstein journal of nanotechnology · 2020Review
- Cytome micronucleus assays with a metabolically competent human derived liver cell line (Huh6): A promising approach for routine testing of chemicals?Environmental and molecular mutagenesis · 2019Article
- Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles.Beilstein journal of nanotechnology · 2019Article
- Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles.Bioengineering (Basel, Switzerland) · 2018Article
- Comparative evaluation of nano and bulk tin dioxide cytotoxicity on dermal fibroblasts by real-time impedance-based and conventional methods.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of a protein corona around nanoparticles can influence their toxicity, triggering cellular responses that may be totally different from those elicited by pristine nanoparticles. The main objective of this study was to investigate whether the species origin of the serum proteins forming the corona influences the in vitro toxicity assessment of silica nanoparticles. Coronas were preformed around nanoparticles before cell exposures by incubation in fetal bovine (FBS) or human (HS) serum. The compositions of these protein coronas were assessed by nano-LC MS/MS. The effects of these protein-coated nanoparticles on HepG2 cells were monitored using real-time cell impedance technology. The nanoparticle coronas formed in human or fetal bovine serum comprised many homologous proteins. Using human compared with fetal bovine serum, nanoparticle toxicity in HepG2 cells decreased by 4-fold and 1.5-fold, when used at 50 and 10μg/mL, respectively. It is likely that "markers of self" are present in the serum and are recognized by human cell receptors. Preforming a corona with human serum seems to be more appropriate for in vitro toxicity testing of potential nanocarriers using human cells. In vitro cytotoxicity assays must reflect in vivo conditions as closely as possible to provide solid and useful results.
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