ArticlePloS one2017
Acid-free glyoxal as a substitute of formalin for structural and molecular preservation in tissue samples.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- DNA Integrity of Chironomids and Oligochaetes Is Maintained During Long-Term Storage in Neutral Buffered Formalin: Implications for Macroinvertebrate Monitoring.Ecology and evolution · 2026Article
- "Green-path": planting seeds for environmental sustainability in diagnostic and research surgical pathology.Virchows Archiv : an international journal of pathology · 2026Review
- First In Vitro Human Islet Assessment of Oleanolic Acid (OA) and Its Serine Conjugate: Enhanced Solubility with Comparable Effects.Molecules (Basel, Switzerland) · 2025Article
- Mass spectrometry-based proteomics of FFPE tissues: progress, limitations, and clinical translation barriers.Clinical proteomics · 2025Review
- Evaluation of Glyoxal fixation for immunohistochemistry of the retina.Scientific reports · 2025Article
- Glyoxal acid-free (GAF) histological fixative is a suitable alternative to formalin: results from an open-label comparative non-inferiority study.Virchows Archiv : an international journal of pathology · 2024Article
- Changes in breast cancer grade from biopsy to excision following surgery or primary chemotherapy.Pathologica · 2024Article
- Fixation in histopathology: the mandate to renew.Pathologica · 2022Article
- Glyoxal Fixation Is Optimal for Immunostaining of Brain Vessels, Pericytes and Blood-Brain Barrier Proteins.International journal of molecular sciences · 2022Article
- Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry.PloS one · 2021Article
- Update of the recommendations for the determination of biomarkers in colorectal carcinoma: National Consensus of the Spanish Society of Medical Oncology and the Spanish Society of Pathology.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2020Article
- PFA is superior to glyoxal in preserving oocyte, embryo, and stem cell proteins evidenced by super-resolution microscopical surveys of epitopes.Journal of assisted reproduction and genetics · 2020Article
- Comparative Analysis of Brain Stiffness Among Amniotes Using Glyoxal Fixation and Atomic Force Microscopy.Frontiers in cell and developmental biology · 2020Article
- Coping with formalin banning in pathology: under vacuum long-term tissue storage with no added formalin.Histochemistry and cell biology · 2019Article
- "Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative.The Analyst · 2019Article
- Safe transportation of formalin-fixed liquid-free pathology specimens.Virchows Archiv : an international journal of pathology · 2018Article
- Effectiveness of different combinations of phenoxetol and formaldehyde on preservation of histological features in human cadaveric tissues.Medical journal, Armed Forces IndiaArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagent. Having observed that the reported drawbacks of commercially available glyoxal substitutes of PBF (Prefer, Glyo-fix, Histo-Fix, Histo-CHOICE, and Safe-Fix II) are likely related to their acidity, we have devised a neutral fixative, obtained by removing acids from the dialdehyde glyoxal with an ion-exchange resin. The resulting glyoxal acid-free (GAF) fixative has been tested in a cohort of 30 specimens including colon (N = 25) and stomach (N = 5) cancers. Our results show that GAF fixation produces a tissue and cellular preservation similar to that produced by PBF. Comparable immuno-histochemical and molecular (DNA and RNA) analytical data were obtained. We observed a significant enrichment of longer DNA fragment size in GAF-fixed compared to PBF-fixed samples. Adoption of GAF as a non-toxic histological fixative of choice would require a process of validation, but the present data suggest that it represents a reliable candidate.
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