ArticleNature protocols2017
Development of fertile mouse oocytes from mitotic germ cells in vitro.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- Ovarian and follicular culture enables the production of fertile oocytes in C57BL/6 mice.The Journal of reproduction and development · 2026Article
- In vitro system completely restores oogenesis in congenitally infertile mice.Human reproduction (Oxford, England) · 2025Article
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolism.iScience · 2024Article
- Induction of Primordial Germ Cell-Like Cells from Rat Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Analysis of Meiotic Progression by Ex Vivo Culture of Mouse Embryonic Ovaries.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Epigenetic regulation limits competence of pluripotent stem cell-derived oocytes.The EMBO journal · 2023Article
- The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture.The Journal of reproduction and development · 2023Article
- In vitro oogenesis from murine premeiotic germ cells using a new three-dimensional culture system.Cell death discovery · 2023Article
- In vitro production of viable eggs from isolated mouse primary follicles by successive culture.The Journal of reproduction and development · 2022Article
- Optimal conditions for mouse follicle culture.The Journal of reproduction and development · 2021Article
- Effect of in vitro growth on mouse oocyte competency, mitochondria and transcriptome.Reproduction (Cambridge, England) · 2021Article
- Blocking estrogen-induced AMH expression is crucial for normal follicle formation.Development (Cambridge, England) · 2021Article
- Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly.Theranostics · 2021Article
- Dissecting the initiation of female meiosis in the mouse at single-cell resolution.Cellular and molecular life sciences : CMLS · 2021Article
- The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries.Scientific reports · 2020Article
- Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development.The Journal of reproduction and development · 2020Article
- The Conundrum of Poor Ovarian Response: From Diagnosis to Treatment.Diagnostics (Basel, Switzerland) · 2020Review
- Review
- Current state and future possibilities of ovarian tissue transplantation.Reproductive medicine and biology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian fetal ovaries contain numerous primordial germ cells (PGCs), although few mature oocytes are obtained from females, owing to apoptosis and follicle atresia. The regulatory mechanisms underlying oogenesis/folliculogenesis remain unknown. Development of methods for obtaining mature oocytes from PGCs in fetal ovaries in vitro could contribute to clarifying these mechanisms. The failure of follicle assembly has been found to be the most challenging aspect in conventional culture conditions. Recently, we established novel culture conditions that enable successful follicle assembly, sustaining interactions between the oocyte and somatic cells, and, in turn, promoting oocyte growth and maturation. Mature oocytes were differentiated from PGCs after a 1-month culture period. A hundred mouse offspring were obtained from approximately a thousand mature oocytes, indicating that oocytes that were differentiated from PGCs in vitro acquired totipotency after fertilization. Here we provide a detailed protocol for using this in vitro system. This in vitro system will potentially provide a novel platform for studying oogenesis and preservation of female germ cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.