ArticleProtein science : a publication of the Protein Society2017
A molecular breadboard: Removal and replacement of subunits in a hepatitis B virus capsid.
Article in Protein science : a publication of the Protein Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Negative cooperativity drives activity of capsid-directed antivirals against hepatitis B virus.Science advances · 2026Article
- Coupling of Size Exclusion Chromatography to High Throughput Charge Detection Mass Spectrometry for the Analysis of Large Proteins and Virus-like Particles.Analytical chemistry · 2025Article
- Chemically Tagging Cargo for Specific Packaging inside and on the Surface of Virus-like Particles.ACS nano · 2024Article
- Article
- Engineering Metastability into a Virus-like Particle to Enable Triggered Dissociation.Journal of the American Chemical Society · 2023Article
- Disassembly of Single Virus Capsids Monitored in Real Time with Multicycle Resistive-Pulse Sensing.Analytical chemistry · 2022Article
- Article
- Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials.Nature communications · 2021Article
- Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry.Nature communications · 2021Article
- The unique potency of Cowpea mosaic virus (CPMV)Biomaterials science · 2020Article
- Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release.Communications biology · 2020Article
- Physical virology: From virus self-assembly to particle mechanics.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- Evolution of Intermediates during Capsid Assembly of Hepatitis B Virus with Phenylpropenamide-Based Antivirals.ACS infectious diseases · 2019Article
- Molecular jenga: the percolation phase transition (collapse) in virus capsids.Physical biology · 2018Article
- Hepatitis B virus core protein allosteric modulators can distort and disrupt intact capsids.eLife · 2018Article
- Geometric Defects and Icosahedral Viruses.Viruses · 2018Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Hepatitis B virus (HBV) core protein is a model system for studying assembly and disassembly of icosahedral structures. Controlling disassembly will allow re-engineering the 120 subunit HBV capsid, making it a molecular breadboard. We examined removal of subunits from partially crosslinked capsids to form stable incomplete particles. To characterize incomplete capsids, we used two single molecule techniques, resistive-pulse sensing and charge detection mass spectrometry. We expected to find a binomial distribution of capsid fragments. Instead, we found a preponderance of 3 MDa complexes (90 subunits) and no fragments smaller than 3 MDa. We also found 90-mers in the disassembly of uncrosslinked HBV capsids. 90-mers seem to be a common pause point in disassembly reactions. Partly explaining this result, graph theory simulations have showed a threshold for capsid stability between 80 and 90 subunits. To test a molecular breadboard concept, we showed that missing subunits could be refilled resulting in chimeric, 120 subunit particles. This result may be a means of assembling unique capsids with functional decorations.
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