ArticleInternational journal of impotence research2017
The effects of miRNA-145 on the phenotypic modulation of rat corpus cavernosum smooth muscle cells.
Article in International journal of impotence research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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10 authors.
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Abstract
To investigate the effect of miR-145 on the phenotypic modulation in rat corpus cavernosum smooth muscle cells. Corpus cavernosum smooth muscle cells were treated with either miR-145 mimics or miR-145 negative control. Cell proliferation were analyzed by the MTS assay and colony formation assay. Wound healing assay were performed to detect the effect of miR-145 on cell migration. The mRNA and protein levels of phenotype marker proteins were assessed by quantitative real-time polymerase chain reaction and western blotting. The intracavernosal pressure and mean arterial pressure were measured to assess erectile function at one month after the injection of platelet-derived growth factor-BB and miR-145. Our results showed that miR-145 inhibited the proliferation and migration of cavernosal smooth muscle cells. Smooth muscle cell phenotypic markers were also affected by overexpression of miR-145, as indicated by the increase in α-smooth muscle actin, calponin and smooth muscle myosin heavy chain expression. Moreover, significantly attenuated erectile function was observed in the platelet-derived growth factor-BB group as compared with the platelet-derived growth factor-BB+miR-145 group. These findings indicated that miR-145 regulate phenotypic modulation of corpus cavernosum smooth muscle cells.
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