ArticleProceedings. Biological sciences2017
Dodging silver bullets: good CRISPR gene-drive design is critical for eradicating exotic vertebrates.
Article in Proceedings. Biological sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 56 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 176 citations in OpenAlex.
- Dissecting the null model for biological invasions: A meta-analysis of the propagule pressure effect.PLoS biology · 2018Pooled it
- Y-linked editors for invasive rodent control.Molecular biology and evolution · 2026Article
- Integrating multiplexing into confineable gene drives effectively overrides resistance in Anopheles stephensi.Nature communications · 2026Article
- Assessing target genes for homing suppression gene drive.The EMBO journal · 2026Article
- Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs.BMC biology · 2025Article
- Maximising Eradication Potential of Rat Gene Drives Using a Two-Target Homing Rescue Strategy: Spatial Modelling of Empirical Data.Molecular ecology · 2025Article
- Controlling the frequency dynamics of homing gene drives for intermediate outcomes.G3 (Bethesda, Md.) · 2025Article
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Population dynamics in spatial suppression gene drive models and the effect of resistance, density dependence, and life history.bioRxiv : the preprint server for biology · 2024Article
- A framework for identifying fertility gene targets for mammalian pest control.Molecular ecology resources · 2024Article
- The suppressive potential of a gene drive in populations of invasive social wasps is currently limited.Scientific reports · 2023Article
- Slow and steady wins the race: Spatial and stochastic processes and the failure of suppression gene drives.Molecular ecology · 2022Article
- A theory of resistance to multiplexed gene drive demonstrates the significant role of weakly deleterious natural genetic variation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles.G3 (Bethesda, Md.) · 2022Article
- Gene drive escape from resistance depends on mechanism and ecology.Evolutionary applications · 2022Article
- Propagation of seminal toxins through binary expression gene drives could suppress populations.Scientific reports · 2022Article
- Modelling homing suppression gene drive in haplodiploid organisms.Proceedings. Biological sciences · 2022Article
- Gene drives for vertebrate pest control: Realistic spatial modelling of eradication probabilities and times for island mouse populations.Molecular ecology · 2022Article
- The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- Generation of Gene Drive Mice for Invasive Pest Population Suppression.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Self-replicating gene drives that can spread deleterious alleles through animal populations have been promoted as a much needed but controversial 'silver bullet' for controlling invasive alien species. Homing-based drives comprise an endonuclease and a guide RNA (gRNA) that are replicated during meiosis via homologous recombination. However, their efficacy for controlling wild populations is threatened by inherent polymorphic resistance and the creation of resistance alleles via non-homologous end-joining (NHEJ)-mediated DNA repair. We used stochastic individual-based models to identify realistic gene-drive strategies capable of eradicating vertebrate pest populations (mice, rats and rabbits) on islands. One popular strategy, a sex-reversing drive that converts heterozygous females into sterile males, failed to spread and required the ongoing deployment of gene-drive carriers to achieve eradication. Under alternative strategies, multiplexed gRNAs could overcome inherent polymorphic resistance and were required for eradication success even when the probability of NHEJ was low. Strategies causing homozygotic embryonic non-viability or homozygotic female sterility produced high probabilities of eradication and were robust to NHEJ-mediated deletion of the DNA sequence between multiplexed endonuclease recognition sites. The latter two strategies also purged the gene drive when eradication failed, therefore posing lower long-term risk should animals escape beyond target islands. Multiplexing gRNAs will be necessary if this technology is to be useful for insular extirpation attempts; however, precise knowledge of homing rates will be required to design low-risk gene drives with high probabilities of eradication success.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.