ArticleJournal of clinical microbiology2017
Neutralization Assay for Zika and Dengue Viruses by Use of Real-Time-PCR-Based Endpoint Assessment.
Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Application of Orthoflavivirus Pseudovirus Technology in Antiviral Research.International journal of molecular sciences · 2026Review
- Neuro-Ophthalmic Dengue Infection: A Case Report with a Multiple Body Site Sampling Strategy and Review of Laboratory Data.Viruses · 2024Article
- Review
- Validation of flavivirus infectious clones carrying fluorescent markers for antiviral drug screening and replication studies.Frontiers in microbiology · 2023Article
- The perspectives of biomarker-based electrochemical immunosensors, artificial intelligence and the Internet of Medical Things toward COVID-19 diagnosis and management.Materials today. Chemistry · 2021Review
- Congenital Zika Virus Infection in a Birth Cohort in Vietnam, 2017-2018.The American journal of tropical medicine and hygiene · 2020Article
- Case Report: Japanese Encephalitis Associated with Chorioretinitis after Short-Term Travel to Bali, Indonesia.The American journal of tropical medicine and hygiene · 2020Article
- A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies.Vaccines · 2020Article
- Immunological Assays used to Support Efficacy of Zika Virus Vaccines.Tropical medicine and infectious disease · 2019Review
- Novel differential linear B-cell epitopes to identify Zika and dengue virus infections in patients.Clinical & translational immunology · 2019Article
- Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies.Virology journal · 2018Article
- Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection.PLoS neglected tropical diseases · 2018Article
- Detection of Zika Virus Infection in Myanmar.The American journal of tropical medicine and hygiene · 2018Article
- Switch-on the LAMP to spot Zika.Annals of translational medicine · 2017Article
- Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The global spread and infective complications of Zika virus (ZKV) and dengue virus (DENV) have made them flaviviruses of public health concern. Serological diagnosis can be challenging due to antibody cross-reactivity, particularly in secondary flavivirus infections or when there is a history of flavivirus vaccination. The virus neutralization assay is considered to be the most specific assay for measurement of anti-flavivirus antibodies. This study describes an assay where the neutralization endpoint is measured by real-time PCR, providing results within 72 h. It demonstrated 100% sensitivity (24/24 ZKV and 15/15 DENV) and 100% specificity (11/11 specimens) when testing well-characterized sera. In addition, the assay was able to determine the correct DENV serotype in 91.7% of cases. The high sensitivity and specificity of the real-time PCR neutralization assay makes it suitable to use as a confirmatory test for sera that are reactive in commercial IgM/IgG enzyme immunoassays. Results are objective and the PCR-based measurement of the neutralization endpoint lends itself to automation so that throughput may be increased in times of high demand.
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