ArticleBMC genetics2017
Investigating knockdown resistance (kdr) mechanism against pyrethroids/DDT in the malaria vector Anopheles funestus across Africa.
Article in BMC genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Four-year monitoring of the malaria vector Anopheles funestus in central-west Cameroon reveals an escalation of pyrethroid resistance combined with high malaria transmission.BMC infectious diseases · 2026Article
- Characterizing the escalation of pyrethroid resistance and its impact on bed nets efficacy alongside molecular basis in Anopheles funestus from Cameroon.Malaria journal · 2025Article
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- Copy number variants underlie major selective sweeps in insecticide resistance genes in Anopheles arabiensis.PLoS biology · 2024Article
- Association of a rapidly selected 4.3kb transposon-containing structural variation with a P450-based resistance to pyrethroids in the African malaria vector Anopheles funestus.PLoS genetics · 2024Article
- Discovery of knock-down resistance in the major African malaria vectorbioRxiv : the preprint server for biology · 2024Article
- Copy number variants underlie the major selective sweeps in insecticide resistance genes inbioRxiv : the preprint server for biology · 2024Article
- Entomological longitudinal surveys in two contrasted eco-climatic settings in Cameroon reveal a high malaria transmission from Anopheles funestus associated with GSTe2 metabolic resistance.BMC infectious diseases · 2023Article
- Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel.Scientific reports · 2023Article
- High efficacy of chlorfenapyr-based net InterceptorInfectious diseases of poverty · 2023Article
- Advances in the genetic characterization of the malaria vector, Anopheles funestus, and implications for improved surveillance and control.Malaria journal · 2023Review
- Escalating pyrethroid resistance in two major malaria vectors Anopheles funestus and Anopheles gambiae (s.l.) in Atatam, Southern Ghana.BMC infectious diseases · 2022Article
- Experimental Hut Trials Reveal That CYP6P9a/b P450 Alleles Are Reducing the Efficacy of Pyrethroid-Only Olyset Net against the Malaria VectorPathogens (Basel, Switzerland) · 2022Article
- First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa.Parasites & vectors · 2021Article
- Insecticide resistance status of Anopheles arabiensis in irrigated and non-irrigated areas in western Kenya.Parasites & vectors · 2021Article
- Potentiation Effects of Ficus sycomorus Active Fraction Against Permethrin-Resistant Field-Population of Anopheles coluzzii (Diptera: Culicidae).Neotropical entomology · 2021Article
- Article
- Assessing cross-resistance within the pyrethroids in terms of their interactions with key cytochrome P450 enzymes and resistance in vector populations.Parasites & vectors · 2021Article
- Towards understanding transfluthrin efficacy in a pyrethroid-resistant strain of the malaria vectorCurrent research in parasitology & vector-borne diseases · 2021Article
- Article
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Abstract
backgroundUnderstanding the molecular basis of insecticide resistance is key to improve the surveillance and monitoring of malaria vector populations under control. In the major malaria vector Anopheles funestus, little is currently known about the role of the knockdown resistance (kdr) mechanism. Here, we investigated the presence and contribution of knockdown resistance (kdr) to pyrethroids/DDT resistance observed in Anopheles funestus across Africa.
resultsPyrosequencing genotyping and sequencing of the voltage gated sodium channel (VGSC) gene did not detect the common L1014F mutation in field collected An. funestus across Africa. Amplification and cloning of the full-length of the sodium channel gene in pyrethroid resistant mosquitoes revealed evidences of alternative splicing events with three transcripts of 2092, 2061 and 2117 amino acids (93% average similarity to An. gambiae). Several amino acid changes were detected close to the domain II of the protein such as L928R, F938 W, I939S, L802S and T1008 M. However, all these mutations are found at low frequency and their role in pyrethroid resistance could not be established. The presence of the exclusive alternative splicing at exon 19 was not associated with resistance phenotype. Analysis of patterns of genetic diversity of the VGSC gene revealed a high polymorphism level of this gene across Africa with no evidence of directional selection suggesting a limited role for knockdown resistance in pyrethroid resistance in An. funestus. Patterns of genetic differentiation correlate with previous observations of the existence of barriers to gene flow Africa-wide with southern population significantly differentiated from other regions.
conclusionDespite an apparent limited role of knockdown resistance in An. funestus, it is necessary to continue to monitor the contribution of the mutations detected here as increasing selection from insecticide-based interventions may change the dynamic in field populations as previously observed in other vectors.
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