ArticleBMC genetics2017
Mapping of leptin and its syntenic genes to chicken chromosome 1p.
Article in BMC genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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15 citing papers in PubMed, 39 citations in OpenAlex.
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- ChickenAnimals : an open access journal from MDPI · 2022Article
- De Novo Assembly of 20 Chicken Genomes Reveals the Undetectable Phenomenon for Thousands of Core Genes on Microchromosomes and Subtelomeric Regions.Molecular biology and evolution · 2022Article
- Sequencing refractory regions in bird genomes are hotspots for accelerated protein evolution.BMC ecology and evolution · 2021Article
- A Novel Hypothalamic Factor, Neurosecretory Protein GM, Causes Fat Deposition in Chicks.Frontiers in physiology · 2021Article
- Evidence for the loss of plasminogen receptor KT gene in chicken.Immunogenetics · 2020Article
- Adipokines Expression and Effects in Oocyte Maturation, Fertilization and Early Embryo Development: Lessons from Mammals and Birds.International journal of molecular sciences · 2020Review
- Expression ofAnimals : an open access journal from MDPI · 2019Article
- Sequence properties of certain GC rich avian genes, their origins and absence from genome assemblies: case studies.BMC genomics · 2019Article
- Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in MammalsInternational journal of molecular sciences · 2019Article
- But where did the centromeres go in the chicken genome models?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Review
- Comparative omics and feeding manipulations in chicken indicate a shift of the endocrine role of visceral fat towards reproduction.BMC genomics · 2018Article
- Chicken Is a Useful Model to Investigate the Role of Adipokines in Metabolic and Reproductive Diseases.International journal of endocrinology · 2018Review
- Correction to: Mapping of leptin and its syntenic genes to chicken chromosome 1p.BMC genetics · 2017Article
- Maternal consumption of fish oil programs reduced adiposity in broiler chicks.Scientific reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 5 institutions in 5 countries.
Funding
Abstract
backgroundMisidentification of the chicken leptin gene has hampered research of leptin signaling in this species for almost two decades. Recently, the genuine leptin gene with a GC-rich (~70%) repetitive-sequence content was identified in the chicken genome but without indicating its genomic position. This suggests that such GC-rich sequences are difficult to sequence and therefore substantial regions are missing from the current chicken genome assembly.
resultsA radiation hybrid panel of chicken-hamster Wg3hCl2 cells was used to map the genome location of the chicken leptin gene. Contrary to our expectations, based on comparative genome mapping and sequence characteristics, the chicken leptin was not located on a microchromosome, which are known to contain GC-rich and repetitive regions, but at the distal tip of the largest chromosome (1p). Following conserved synteny with other vertebrates, we also mapped five additional genes to this genomic region (ARF5, SND1, LRRC4, RBM28, and FLNC), bridging the genomic gap in the current Galgal5 build for this chromosome region. All of the short scaffolds containing these genes were found to consist of GC-rich (54 to 65%) sequences comparing to the average GC-content of 40% on chromosome 1. In this syntenic group, the RNA-binding protein 28 (RBM28) was in closest proximity to leptin. We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detecting a negative correlation (R = - 0.7) between the expression of leptin and of RBM28 across tissues that expressed at least one of the genes above the average level. This observation suggested a local regulatory interaction between these genes. In adipose tissues, we observed a significant increase in RBM28 mRNA expression in breeds with lean phenotypes.
conclusionMapping chicken leptin together with a cluster of five syntenic genes provided the final proof for its identification as the true chicken ortholog. The high GC-content observed for the chicken leptin syntenic group suggests that other similar clusters of genes in GC-rich genomic regions are missing from the current genome assembly (Galgal5), which should be resolved in future assemblies of the chicken genome.
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