Evidence map›Paper›PMID 28793248›Full record

ArticleStem cell reports2017

Replicable Expansion and Differentiation of Neural Precursors from Adult Canine Skin.

Thomas Duncan, Aileen Lowe, Kuldip Sidhu, Perminder Sachdev, Trevor Lewis, Ruby C Y Lin, Vladimir Sytnyk, Michael Valenzuela

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Thomas DuncanRegenerative Neuroscience Group, Brain and Mind Centre, University of Sydney, Sydney, NSW 2050, Australia.
Aileen LoweRegenerative Neuroscience Group, Brain and Mind Centre, University of Sydney, Sydney, NSW 2050, Australia; Stem Cell Laboratory, University of New South Wales, Sydney, NSW 2031, Australia.
Kuldip SidhuStem Cell Laboratory, University of New South Wales, Sydney, NSW 2031, Australia.
Perminder SachdevCentre for Healthy Brain Ageing, University of New South Wales, Sydney, NSW 2031, Australia; School of Psychiatry, University of New South Wales, Sydney, NSW 2052, Australia.
Trevor LewisSchool of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Ruby C Y LinSchool of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Vladimir SytnykSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW 2052, Australia.
Michael ValenzuelaRegenerative Neuroscience Group, Brain and Mind Centre, University of Sydney, Sydney, NSW 2050, Australia. Electronic address: michael.valenzuela@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Repopulation of brain circuits by neural precursors is a potential therapeutic strategy for neurodegenerative disorders; however, choice of cell is critical. Previously, we introduced a two-step culture system that generates a high yield of neural precursors from small samples of adult canine skin. Here, we probe their gene and protein expression profiles in comparison with dermal fibroblasts and brain-derived neural stem cells and characterize their neuronal potential. To date, we have produced >50 skin-derived neural precursor (SKN) lines. SKNs can be cultured in a highly replicable fashion and uniformly express a panel of identifying markers. Upon differentiation, they self-upregulate neural specification genes, generating neurons with basic electrophysiological functionality. This unique population of neural precursors, derived from mature skin, overcomes many of the practical issues that have limited clinical translation of alternative cell types. Easily accessible, neuronally committed, and patient specific, SKNs may have potential for the treatment of brain disorders.

Indexed as

Cell DifferentiationAnimalsBiomarkersCell Culture TechniquesCell ProliferationCells, CulturedDogsGene Expression ProfilingImmunophenotypingNeural Stem CellsPhenotypeSkinSynapsesBiomarkersadult stem cellsdifferentiationneural precursorsneurogenicneuronsskinSKN

Identifiers

PMID28793248
PMCPMC5550271

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.