ArticleClinics (Sao Paulo, Brazil)2017
Validation of QF-PCR for prenatal diagnoses in a Brazilian population.
Article in Clinics (Sao Paulo, Brazil), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Evaluating the Effectiveness of Quantitative Fluorescent Polymerase Chain Reaction as a Substitute or Complement to Conventional Karyotyping for Prenatal Diagnosis.Journal of obstetrics and gynaecology of India · 2025Article
- The Impact of Chromosomal Mosaicisms on Prenatal Diagnosis and Genetic Counseling-A Narrative Review.Journal of personalized medicine · 2024Review
- Article
- Detection of partial deletion and mosaicism using quantitative fluorescent polymerase chain reaction: Case reports and a review of the literature.Journal of clinical laboratory analysis · 2022Review
- Development and validation of a novel 26-plex system for prenatal diagnosis with forensic markers.International journal of legal medicine · 2022Article
- Molecular karyotypes of loquat (Plant methods · 2020Article
- Graft versus host disease and microchimerism in aAllergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2019Article
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Authors and funding
7 authors.
Funding
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Abstract
objectives: Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services. We investigated the accuracy of QF-PCR for the prenatal recognition of common aneuploidies and compared these results with cytogenetic results in our laboratory.
method: A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes.
results: A total of 162 amniotic fluid samples analyzed using multiplex QF-PCR were compared with karyotyping analysis. The QF-PCR results were consistent with the results of cytogenetic analysis in 95.4% of all samples.
conclusion: QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidy screening. This study suggests that QF-PCR can be used as a rapid diagnostic method. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis.
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