ArticleEquine veterinary journal2018
Advanced three-dimensional culture of equine intestinal epithelial stem cells.
Article in Equine veterinary journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Functional and molecular characterization of equine intestinal organoids across media conditions and intestinal segments.BMC veterinary research · 2026Article
- Equine coronavirus infection and replication in equine intestinal enteroids.Veterinary research · 2024Article
- A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium.Veterinary research · 2024Article
- Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection.Veterinary research · 2024Article
- Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm.International journal of molecular sciences · 2024Review
- Assessment of equine intestinal epithelial junctional complexes and barrier permeability using a monolayer culture system.Frontiers in veterinary science · 2024Article
- Accessible luminal interface of bovine rectal organoids generated from cryopreserved biopsy tissues.PloS one · 2024Article
- Companion animal organoid technology to advance veterinary regenerative medicine.Frontiers in veterinary science · 2023Review
- Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications.BMC veterinary research · 2022Article
- Establishment of intestinal organoids from small intestine of growing cattle (12 months old).Journal of animal science and technology · 2022Article
- Low Flow versus No Flow: Ischaemia Reperfusion Injury Following Different Experimental Models in the Equine Small Intestine.Animals : an open access journal from MDPI · 2022Article
- Intestinal Models for Personalized Medicine: from Conventional Models to Microfluidic Primary Intestine-on-a-chip.Stem cell reviews and reports · 2022Review
- Establishment of bovine 3D enteroid-derived 2D monolayers.Veterinary research · 2022Article
- Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans.Frontiers in medical technology · 2022Review
- Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer.Veterinary research · 2021Article
- Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics.Veterinary research · 2021Article
- Organoids: a promising new in vitro platform in livestock and veterinary research.Veterinary research · 2021Review
- Culture and differentiation of rabbit intestinal organoids and organoid-derived cell monolayers.Scientific reports · 2021Article
- Intestinal organoids in farm animals.Veterinary research · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIntestinal epithelial stem cells are critical to epithelial repair following gastrointestinal injury. The culture of intestinal stem cells has quickly become a cornerstone of a vast number of new research endeavours that range from determining tissue viability to testing drug efficacy for humans. This study aims to describe the methods of equine stem cell culture and highlights the future benefits of these techniques for the advancement of equine medicine.
objectivesTo describe the isolation and culture of small intestinal stem cells into three-dimensional (3D) enteroids in horses without clinical gastrointestinal abnormalities. STUDY
designDescriptive study.
methodsIntestinal samples were collected by sharp dissection immediately after euthanasia. Intestinal crypts containing intestinal stem cells were dissociated from the underlying tissue layers, plated in a 3D matrix and supplemented with growth factors. After several days, resultant 3D enteroids were prepared for immunofluorescent imaging and polymerase chain reaction (PCR) analysis to detect and characterise specific cell types present. Intestinal crypts were cryopreserved immediately following collection and viability assessed.
resultsIntestinal crypts were successfully cultured and matured into 3D enteroids containing a lumen and budding structures. Immunofluorescence and PCR were used to confirm the existence of stem cells and all post mitotic, mature cell types, described to exist in the horse intestinal epithelium. Previously frozen crypts were successfully cultured following a freeze-thaw cycle. MAIN LIMITATIONS: Tissues were all derived from normal horses. Application of this technique for the study of specific disease was not performed at this time.
conclusionsThe successful culture of equine intestinal crypts into 3D "mini-guts" allows for in vitro studies of the equine intestine. Additionally, these results have relevance to future development of novel therapies that harness the regenerative potential of equine intestine in horses with gastrointestinal disease (colic).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.