Evidence map›Paper›PMID 28792150›Full record

ArticleYonsei medical journal2017

Immune Tolerance of Human Dental Pulp-Derived Mesenchymal Stem Cells Mediated by CD4⁺CD25⁺FoxP3⁺ Regulatory T-Cells and Induced by TGF-β1 and IL-10.

Jong Won Hong, Jung Hyun Lim, Chooryung J Chung, Tae Jo Kang, Tae Yeon Kim, Young Seok Kim, Tae Suk Roh, Dae Hyun Lew

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Yonsei medical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 72 citations in OpenAlex.

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  4. Stem cell therapy for regenerating periodontal bony defects: A narrative review.Journal of advanced periodontology & implant dentistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jong Won HongDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Jung Hyun LimDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Chooryung J ChungDepartment of Orthodontics, College of Dentistry, Yonsei University, Seoul, Korea.
Tae Jo KangDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Tae Yeon KimDepartment of Orthodontics, College of Dentistry, Yonsei University, Seoul, Korea.
Young Seok KimDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Tae Suk RohDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Dae Hyun LewDepartment of Plastic & Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea.
Yonsei University · KRPlastic Surgery Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeMost studies on immune tolerance of mesenchymal stem cells (MSCs) have been performed using MSCs derived from bone marrow, cord blood, or adipose tissue. MSCs also exist in the craniofacial area, specifically in teeth. The purpose of this study was to evaluate the mechanisms of immune tolerance of dental pulp-derived MSC (DP-MSC) in vitro and in vivo. MATERIALS AND

methodsWe isolated DP-MSCs from human dental pulp and co-cultured them with CD4⁺ T-cells. To evaluate the role of cytokines, we blocked TGF-β and IL-10, separately and together, in co-cultured DP-MSCs and CD4⁺ T-cells. We analyzed CD25 and FoxP3 to identify regulatory T-cells (Tregs) by fluorescence-activated cell sorting (FACS) and real-time PCR. We performed alloskin grafts with and without DP-MSC injection in mice. We performed mixed lymphocyte reactions (MLRs) to check immune tolerance.

resultsCo-culture of CD4⁺ T-cells with DP-MSCs increased the number of CD4⁺CD25⁺FoxP3⁺ Tregs (p<0.01). TGF-β or/and IL-10 blocking suppressed Treg induction in co-cultured cells (p<0.05). TGF-β1 mRNA levels were higher in co-cultured DP-MSCs and in co-cultured CD4⁺ T-cells than in the respective monocultured cells. However, IL-10 mRNA levels were not different. There was no difference in alloskin graft survival rate and area between the DP-MSC injection group and the non-injection group. Nonetheless, MLR was reduced in the DP-MSC injected group (p<0.05).

conclusionDP-MSCs can modulate immune tolerance by increasing CD4⁺CD25⁺FoxP3⁺ Tregs. TGF-β1 and IL-10 are factors in the immune-tolerance mechanism. Pure DP-MSC therapy may not be an effective treatment for rejection, although it may module immune tolerance in vivo.

Indexed as

Immune ToleranceAllograftsAnimalsBiomarkersCD4 AntigensCell ProliferationCoculture TechniquesDental PulpForkhead Transcription FactorsGraft SurvivalHumansInterleukin-10Interleukin-2 Receptor alpha SubunitLymphocyte Culture Test, MixedMesenchymal Stem CellsMesenchymal Stem Cell TransplantationBiomarkersCD4 AntigensForkhead Transcription FactorsFOXP3 protein, humanInterleukin-10Interleukin-2 Receptor alpha SubunitTransforming Growth Factor beta1Dental pulpimmune tolerancemesenchymal stem cell

Identifiers

PMID28792150
PMCPMC5552631
OpenAlexW2743601076

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.