Evidence map›Paper›PMID 28791211›Full record

Article3 Biotech2017

Development and characterization of polymorphic EST based SSR markers in barley (

Won-Sam Jo, Hye-Yeong Kim, Kyung-Min Kim

Abstract read
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Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Won-Sam JoSchool of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 41566 Korea.
Hye-Yeong KimSchool of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 41566 Korea.
Kyung-Min KimSchool of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 41566 Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In barley, breeding using good genetic characteristics can improve the quality or quantity of crop characters from one generation to the next generation. The development of effective molecular markers in barley is crucial for understanding and analyzing the diversity of useful alleles. In this study, we conducted genetic relationship analysis using expressed sequence tag-simple sequence repeat (EST-SSR) markers for barley identification and assessment of barley cultivar similarity. Seeds from 82 cultivars, including 31 each of naked and hulled barley from the Korea Seed and Variety Service and 20 of malting barley from the RDA-Genebank Information Center, were analyzed in this study. A cDNA library of the cultivar Gwanbori was constructed for use in analysis of genetic relationships, and 58 EST-SSR markers were developed and characterized. In total, 47 SSR markers were employed to analyze polymorphisms. A relationship dendrogram based on the polymorphism data was constructed to compare genetic diversity. We found that the polymorphism information content among the examined cultivars was 0.519, which indicates that there is low genetic diversity among Korean barley cultivars. The results obtained in this study may be useful in preventing redundant investment in new cultivars and in resolving disputes over seed patents. Our approach can be used by companies and government groups to develop different cultivars with distinguishable markers. In addition, the developed markers can be used for quantitative trait locus analysis to improve both the quantity and the quality of cultivated barley.

Indexed as

BarleyDendrogramEST-SSR markerGenetic similarity

Identifiers

PMID28791211
PMCPMC5529294

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