ArticleScientific reports2017
Unraveling heme detoxification in the malaria parasite by in situ correlative X-ray fluorescence microscopy and soft X-ray tomography.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 84 citations in OpenAlex.
- Artificial intelligence-powered microscopy: Transforming the landscape of parasitology.Journal of microscopy · 2026Review
- Demonstrating soft X-ray tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy.Scientific reports · 2025Article
- Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.Chemical & biomedical imaging · 2024Article
- Artemisinin-resistant Plasmodium falciparum Kelch13 mutant proteins display reduced heme-binding affinity and decreased artemisinin activation.Communications biology · 2024Article
- Cryo-tomography and 3D Electron Diffraction Reveal the Polar Habit and Chiral Structure of the Malaria Pigment Crystal Hemozoin.ACS central science · 2024Article
- Nonclassical mechanisms to irreversibly suppress β-hematin crystal growth.Communications biology · 2023Article
- The vacuolar iron transporter mediates iron detoxification in Toxoplasma gondii.Nature communications · 2023Article
- 3D surface reconstruction of cellular cryo-soft X-ray microscopy tomograms using semisupervised deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- High-efficiency focusing and imaging by dielectric kinoform zone plate lenses with soft X-rays.Journal of synchrotron radiation · 2023Article
- Adsorption to the Surface of Hemozoin Crystals: Structure-Based Design and Synthesis of Amino-Phenoxazine β-Hematin Inhibitors.ChemMedChem · 2022Article
- Disrupting Crystal Growth through Molecular Recognition: Designer Therapies for Kidney Stone Prevention.Accounts of chemical research · 2022Article
- Reactive Oxygen Species as the Brainbox in Malaria Treatment.Antioxidants (Basel, Switzerland) · 2021Review
- Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.Nature protocols · 2021Article
- Malaria Pigment Crystals: The Achilles' Heel of the Malaria Parasite.ChemMedChem · 2021Review
- Oxidation of Erythrocytes Enhance the Production of Reactive Species in the Presence of Artemisinins.International journal of molecular sciences · 2020Article
- Mode of action of quinoline antimalarial drugs in red blood cells infected byProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Antimalarial Quinoline Drugs Inhibit β-Hematin and Increase Free Hemin Catalyzing Peroxidative Reactions and Inhibition of Cysteine Proteases.Scientific reports · 2019Article
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A key drug target for malaria has been the detoxification pathway of the iron-containing molecule heme, which is the toxic byproduct of hemoglobin digestion. The cornerstone of heme detoxification is its sequestration into hemozoin crystals, but how this occurs remains uncertain. We report new results of in vivo rate of heme crystallization in the malaria parasite, based on a new technique to measure element-specific concentrations at defined locations in cell ultrastructure. Specifically, a high resolution correlative combination of cryo soft X-ray tomography has been developed to obtain 3D parasite ultrastructure with cryo X-ray fluorescence microscopy to measure heme concentrations. Our results are consistent with a model for crystallization via the heme detoxification protein. Our measurements also demonstrate the presence of considerable amounts of non-crystalline heme in the digestive vacuole, which we show is most likely contained in hemoglobin. These results suggest a tight coupling between hemoglobin digestion and heme crystallization, highlighting a new link in the crystallization pathway for drug development.
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